We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?

Name: beta Tubulin Antibody
Brand: Novus Biologicals
SKU: NB100-56459
Price: 399 USD

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Accepted Answer

According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, Pm, XpSpecies Glossary
Applications	WB, Simple Western, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Immunogen	A synthetic peptide corresponding to a portion of amino acids 400-450 of human b-tubulin was used as immunogen for this antibody, GenBank no. gi\|27227551\|gb\|AAN85571.1\|.
Specificity	The amino acid sequence used as immunogen has 100% identity in beta I and beta II tubulin.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	TUBB
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein G purified

Publications using NB100-56459	Applications	Species
Cheramangalam RN, Anand T, Pandey P et al. Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRC PLoS genetics 2023-04-01 [PMID: 37098042] (WB, Drosophila)	WB	Drosophila
Lima C, Andrade-Barros AI, Bernardo JTG et al. Natterin-Induced Neutrophilia Is Dependent on cGAS/STING Activation via Type I IFN Signaling Pathway International journal of molecular sciences [PMID: 35408954] (WB, Mouse)	WB	Mouse
Krisko TI, Nicholls HT, Bare CJ et al. Dissociation of Adaptive Thermogenesis from Glucose Homeostasis in Microbiome-Deficient Mice Cell Metab. 2020-02-12 [PMID: 32084379]
R Goulet C, Bernard G, Tremblay S et al. Exosomes Induce Fibroblast Differentiation into Cancer-associated Fibroblasts through TGFb Signaling. Mol. Cancer Res. 2018-04-10 [PMID: 29636362] (WB)	WB
Chen M, Arumugam TV, Leanage G et al. Disease-modifying effect of intravenous immunoglobulin in an experimental model of epilepsy Sci Rep. 2017-01-11 [PMID: 28074934] (WB, Mouse)	WB	Mouse
Williams K, Balsor JL, Beshara S et al. Experience-dependent central vision deficits: Neurobiology and visual acuity Vision Res. 2015-02-07 [PMID: 25668772] (WB, Feline) Details: beta-Tubulin antibody used at 1:4000 dilution in WB application for analysing 25ug lysates of the central, peripheral, and monocular regions of visual cortex /V1 section obtained from the brain tissues of 5 week old Feline/Cats which were subjected to 6 hours to 7 days of monocular deprivation (Fig. 1).	WB	Feline
Murphy KM, Balsor J, Beshara S et al. A high-throughput semi-automated preparation for filtered synaptoneurosomes J. Neurosci. Methods. 2014-07-02 [PMID: 24997341] (WB, Canine, Rat) Details: beta Tubulin antibody used at 1:4000 dilution in WB on whole homogenate and synaptoneurosomes from somatosensory cortex of Long Evans rats and adult cat/feline frontal cortex.	WB	Canine, Rat
Saha T. LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy. Autophagy. 2012-11-01 [PMID: 22874552]
Li R, Guan H, Yang K. Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes. Regul Pept. 2012-10-10 [PMID: 22750277]
Kamal A, Sreekanth K, Kumar PP et al. Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines. Eur J Med Chem. 2010-06-01 [PMID: 20171761] Details: Products cited for western blot: 1. PARP (IMG-401A): MCF-7 cells treated with numerous compounds, Fig 7. 2. B-Tubulin (IMG-5180A): MCF-7 cells treated with PP-PBDs, Fig 6 and Fig 7. 3. Cytochrome C (IMG-101A): MCF-7 cells treated with numerous compounds,
Boniface K, Diveu C, Morel F et al. Oncostatin M secreted by skin infiltrating T lymphocytes is a potent keratinocyte activator involved in skin inflammation. J Immunol. 2007-04-01 [PMID: 17372020]
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beta Tubulin Antibody

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beta II Tubulin A Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)

Gene Symbol	TUBB
Entrez	7280(Human) 22151(Mouse) 498736(Rat)
Uniprot	P07437(Human)