![ELISA: Human beta-Actin ELISA Kit (Colorimetric) [NBP3-27508] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27508_human-beta-actin-elisa-kit-colorimetric-114202410253622.png "ELISA: Human beta-Actin ELISA Kit (Colorimetric) [NBP3-27508] - Standard Curve Reference")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids |
| Standard Curve Range | 1.56 - 100 ng/mL (example only; lot dependent) |
| Sensitivity | 0.63 ng/mL (example only; lot dependent) |
| Description | Assay Length: 4.5 hours |
| Standard Curve Range | 1.56 - 100 ng/mL (example only; lot dependent) |
| Sensitivity | 0.63 ng/mL (example only; lot dependent) |
| Assay Type | Sandwich ELISA |
| Inter-Assay | %CV < 12 (example only; lot dependent) |
| Intra-Assay | %CV < 10 (example only; lot dependent) |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | ACTB |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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| Gene Symbol | ACTB |
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![Flow Cytometry: beta-Actin Antibody [NB600-503] - An intracellular stain was performed on HeLa cells with beta-actin Antibody NB600-503AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](https://images.novusbio.com/images/beta-Actin-Antibody-Flow-Cytometry-NB600-503-img0013.jpg "Flow Cytometry: beta-Actin Antibody [NB600-503] - An intracellular stain was performed on HeLa cells with beta-actin Antibody NB600-503AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")