| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF |
| Clone | 937215 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Peptide containing a sequence at the N-terminus of human beta ‑Actin Accession # P60709 |
| Specificity | Detects human, mouse, and rat beta ‑Actin in Western blots. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACTB |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
Simple Western | Human | 08/27/2020 |
Summary
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Enlarge |
reviewed by:
Dahao Ling |
Simple Western | Human | 11/07/2019 |
Summary
Comments
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Enlarge |
reviewed by:
Verified Customer |
WB | Human | 03/15/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
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Application Focus: I see an increase in LC3, now what? By Christina Towers, PhD. Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm... Read full blog post. |
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
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Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post. |
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ACTB - an abundant cytoskeletal component with applications for gene expression analysis Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi... Read full blog post. |
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A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 08/27/2020 |
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| Application: | Simple Western | |
| Species: | Human |
| Dahao Ling 11/07/2019 |
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| Application: | Simple Western | |
| Species: | Human |
| Verified Customer 03/15/2016 |
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| Application: | WB | |
| Species: | Human |
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