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beta 2-Microglobulin Antibody (893803) [Unconjugated]

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Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse beta 2-Microglobulin Monoclonal Antibody (Catalog # MAB8325) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for beta 2-Microglobulin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
893803
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8325-SP
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beta 2-Microglobulin Antibody (893803) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse beta 2‑Microglobulin
Met1-Met119
Accession # P01887
Specificity
Detects mouse  beta 2‑Microglobulin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
B2M
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB8325 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta 2-Microglobulin Antibody (893803) [Unconjugated]

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta 2-Microglobulin (b2M) is a 12 kDa secreted polypeptide that serves as the light chain of Class I MHC molecules. Possessing an Ig-like domain, b2M noncovalently associates with both 44 kDa classical (HLA-A, -B, -C) and 40 kDa non-classical (HLA-E, -F, -G) Class I MHC heavy chains as well as with 43-49 kDa Class I non-MHC heavy chains (CD1). b2M is expressed on nearly all nucleated cells, with neurons being a notable exception. Circulating b2M is generated during normal HLA turnover. It can also dissociate from the MHC complex and circulate as full length and N-terminal truncated peptides of 93, 91, and 90 amino acids. It has been measured in a variety of body fluids, including serum, plasma, saliva, CSF, and urine. b2M freely passes through the glomerular membrane, but it is 99% actively reabsorbed and degraded in the proximal tubule cells. Circulating b2M levels are elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions with decreased glomerular filtration. Human b2M shares 70% and 75% amino acid sequence identity with mouse and rat b2M, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta 2-Microglobulin Antibody (MAB8325)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol B2M
Uniprot