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BACE-2 Antibody

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Western Blot: BACE2 Antibody [NBP2-15539] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: BACE2 Antibody [NBP2-15539] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: BACE2 Antibody [NBP2-15539] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: BACE-2 Antibody [NBP2-15539] - : Paraffin-embedded human breast carcinoma. BACE2 antibody [C1C3] dilution: 1:500.
Immunohistochemistry-Paraffin: BACE-2 Antibody [NBP2-15539] - Paraffin-embedded Rat brain. BACE2 antibody [C1C3] diluted at 1:500.
Immunohistochemistry-Paraffin: BACE-2 Antibody [NBP2-15539] - Paraffin-embedded rat fore brain. BACE2 antibody [C1C3] dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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BACE-2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human BACE-2. The exact sequence is proprietary.
Localization
Membrane
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BACE2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for BACE-2 Antibody

  • AEPLC
  • Asp 1
  • ASP1
  • ASP21
  • Aspartic-like protease 56 kDa
  • Aspartyl protease 1
  • BACE2
  • BACE-2
  • beta secretase 2
  • beta-secretase 2
  • beta-site amyloid beta A4 precursor protein-cleaving enzyme 2
  • Beta-site amyloid precursor protein cleaving enzyme 2
  • Beta-site APP cleaving enzyme 2
  • beta-site APP-cleaving enzyme 2
  • beta-site APP-cleaving enzyme 2, EC 3.4.2310ALP5656 kDa aspartic-like protease
  • CEAP1
  • Down region aspartic protease
  • Down syndrome region aspartic protease
  • DRAPBAE2
  • EC 3.4.23
  • EC 3.4.23.45
  • memapsin-1
  • Membrane-associated aspartic protease 1
  • theta-secretase
  • transmembrane aspartic proteinase Asp1

Background

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease and a frequent complication of Down syndrome. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein by 2 proteases, one of which is the protein encoded by this gene. This gene localizes to the 'Down critical region' of chromosome 21. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BACE2
Uniprot