![Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-Western-Blot-NB500-185-img0006.jpg "Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 344) of human Aurora B kinase using the numbering given in Swiss-Prot entry Q96GD4 (GeneID 9212). |
| Marker | Mitosis Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AURKB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB500-185] - Asynchronous HeLa cells. Antibody used at 0.5 ug/ml in A and at 2 ug/ml in B. Detection : Secondary antibodies were anti-Rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-Immunocytochemistry-Immunofluorescence-NB500-185-img0004.jpg "Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB500-185] - Asynchronous HeLa cells. Antibody used at 0.5 ug/ml in A and at 2 ug/ml in B. Detection : Secondary antibodies were anti-Rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.")
![Immunoprecipitation: Aurora B Antibody [NB500-185] - Detection of human Aurora B by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 (lot NB500-185-2) used at 0.1 ug/ml. Aurora B was also immunoprecipitated by a previous lot of this antibody (lot NB500-185-1). Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-Immunoprecipitation-NB500-185-img0007.jpg "Immunoprecipitation: Aurora B Antibody [NB500-185] - Detection of human Aurora B by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 (lot NB500-185-2) used at 0.1 ug/ml. Aurora B was also immunoprecipitated by a previous lot of this antibody (lot NB500-185-1). Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")