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Aurora B Antibody (2B1) - BSA Free

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Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins ...read more
Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clone
2B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Aurora B Antibody (2B1) - BSA Free Summary

Immunogen
Recombinant human Aurora B (1-344aa) purified from E. coli
Marker
Mitosis Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AURKB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Aurora B Antibody (2B1) - BSA Free

  • Aik2
  • AIK2EC 2.7.11.1
  • AIM1
  • AIM-1
  • AIM1Aik2
  • AIM-1STK-1
  • ARK2
  • ARK2ARK-2
  • AurB
  • AURKB
  • aurkb-sv2
  • Aurora- and IPL1-like midbody-associated protein 1
  • Aurora B
  • aurora kinase BSerine/threonine-protein kinase aurora-B
  • aurora kinase B-Sv1
  • aurora kinase B-Sv2
  • Aurora/IPL1-related kinase 2
  • aurora-1
  • aurora-B
  • Aurora-related kinase 2
  • EC 2.7.11
  • IPL1
  • serine/threonine kinase 12
  • serine/threonine-protein kinase 12
  • STK12
  • STK12aurkb-sv1
  • STK5

Background

The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AURKB
Uniprot