![Western Blot: Aurora A Antibody [NB100-267] - Samples: Whole cell lysate (50 ug) from HeLa and HEPG2cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NB100-267-img0005.jpg "Western Blot: Aurora A Antibody [NB100-267] - Samples: Whole cell lysate (50 ug) from HeLa and HEPG2cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790). |
| Marker | Mitosis Marker |
| Specificity | This is specific for human STK15, Serine/Threonine Kinase 15, Aurora 2, BRAK, ARK1, Aurora-Related Kinase 1 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AURKA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Human Pancreas Whole Tissue Lysate (Adult Whole Tumor) | |
| MCF-7 Whole Cell Lysate | |
| Aurora A Overexpression Lysate |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aurora A Antibody (NB100-267)Find related products by research area.
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![Immunoprecipitation: Aurora A Antibody [NB100-267] - Detection of human Aurora A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aurora A antibody NB100-267 used for IP at 3 ug per reaction. Aurora A was also immunoprecipitated by another rabbit anti-Aurora A antibody. For blotting immunoprecipitated Aurora A, NB100-267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunoprecipitation-NB100-267-img0006.jpg "Immunoprecipitation: Aurora A Antibody [NB100-267] - Detection of human Aurora A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aurora A antibody NB100-267 used for IP at 3 ug per reaction. Aurora A was also immunoprecipitated by another rabbit anti-Aurora A antibody. For blotting immunoprecipitated Aurora A, NB100-267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")