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Aurora A Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora A at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aurora A at approximately 56 kDa (as indicated) using 12.5 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF3295
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3295-SP
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Aurora A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Aurora A
Met1-Glu131
Accession # O14965
Specificity
Detects human Aurora A in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AURKA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 12.5 ug/mL
  • Western Blot 0.25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF3295 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aurora A Antibody [Unconjugated]

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Background

Aurora A is a serine/threonine kinase that regulates chromosome segregation and cytokinesis during mitosis. Its kinase activity is thought to regulate the G2 to M transition of the cell cycle by phosphorylating BRCA1. Aberrant expression and activity of Aurora A in tumors, including breast, ovarian, gastric and colorectal cancer, suggests that it plays a role in tumor development and progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (AF3295)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(3)
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Human
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF3295 Applications Species
Yung-Chieh Chang, Chun Hei Antonio Cheung, Yao-Lung Kuo Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer Cells Frontiers in Cell and Developmental Biology 6/30/2020 [PMID: 32695778]
Seth A. Wander, Ofir Cohen, Xueqian Gong, Gabriela N. Johnson, Jorge E. Buendia-Buendia, Maxwell R. Lloyd et al. The Genomic Landscape of Intrinsic and Acquired Resistance to Cyclin-Dependent Kinase 4/6 Inhibitors in Patients with Hormone Receptor–Positive Metastatic Breast Cancer Cancer Discovery 8/1/2020 [PMID: 32404308]
J Du, L Yan, R Torres, X Gong, H Bian, C Marugán, K Boehnke, C Baquero, YH Hui, SC Chapman, Y Yang, Y Zeng, SM Bogner, RT Foreman, A Capen, GP Donoho, RD Van Horn, DS Barnard, JA Dempsey, RP Beckmann, MS Marshall, LC Chio, Y Qian, YW Webster, A Aggarwal, S Chu, S Bhattachar, LF Stancato, MS Dowless, PW Iversen, JR Manro, JL Walgren, BW Halstead, MZ Dieter, R Martinez, SV Bhagwat, EL Kreklau, MJ Lallena, XS Ye, BKR Patel, C Reinhard, GD Plowman, DA Barda, JR Henry, SG Buchanan, RM Campbell Aurora A selective inhibitor LY3295668 leads to dominant mitotic arrest, apoptosis in cancer cells and shows potent preclinical antitumor efficacy Mol. Cancer Ther., 2019-09-17;0(0):. 2019-09-17 [PMID: 31530649] (ICC, Western Blot, Human) ICC, Western Blot Human
X Gong, J Du, SH Parsons, FF Merzoug, Y Webster, PW Iversen, LC Chio, RD Van Horn, X Lin, W Blosser, B Han, S Jin, S Yao, H Bian, C Ficklin, L Fan, A Kapoor, S Antonysamy, AM Mc Nulty, K Froning, D Manglicmot, A Pustilnik, K Weichert, SR Wasserman, M Dowless, C Marugán, C Baquero, MJ Lallena, SW Eastman, YH Hui, MZ Dieter, T Doman, S Chu, HR Qian, XS Ye, DA Barda, GD Plowman, C Reinhard, RM Campbell, JR Henry, SG Buchanan Aurora-A kinase inhibition is synthetic lethal with loss of the RB1 tumor suppressor gene Cancer Discov, 2018-10-29;0(0):. 2018-10-29 [PMID: 30373917] (Western Blot, Human) Western Blot Human
Tiedemann RE, Zhu YX, Schmidt J, Yin H, Shi CX, Que Q, Basu G, Azorsa D, Perkins LM, Braggio E, Fonseca R, Bergsagel PL, Mousses S, Stewart AK Kinome-wide RNAi studies in human multiple myeloma identify vulnerable kinase targets, including a lymphoid-restricted kinase, GRK6. Blood, 2009-12-07;115(8):1594-604. 2009-12-07 [PMID: 19996089] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol AURKA
Uniprot