![ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Samples were spiked with high concentrations of Human ATM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69891_human-atm-elisa-kit-colorimetric-132202416133650.jpg "ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Samples were spiked with high concentrations of Human ATM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 0.16 - 10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Specificity | Human, ATM ELISA Kit (Colorimetric) recognizes Human ATM in samples. No significant cross-reactivity or interference between Human ATM and analogues was observed. |
| Standard Curve Range | 0.16 - 10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | CV% < 4.93% |
| Intra-Assay | CV% < 5.19% |
| Spike Recovery | 87-106% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | ATM |
| Dilutions |
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| Publications |
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| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Simple Western ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Simple_Western_20109.jpg)
Research Areas for ATM ELISA Kit (NBP2-69891)Find related products by research area.
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Further unraveling the role of gamma H2AX in DNA damage response Our genome experiences a moderate amount of DNA damage in our cells on a daily basis. This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry. While normal rates of DNA ... Read full blog post. |
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The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post. |
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Application Highlight: Recent uses of TERF2 in immunofluorescence (IF) Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion. TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th... Read full blog post. |
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ATM - detecting and responding to DNA damage Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels ... Read full blog post. |
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53BP1 - a marker for DNA Double Strand Break 53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response... Read full blog post. |
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53BP1 - DNA damage is no fun The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri... Read full blog post. |
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ATM and DSB Repair in Cancer Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to... Read full blog post. |
| Gene Symbol | ATM |
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![Knockdown Validated: ATM Antibody (2C1) [NB100-309] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:500.](https://images.novusbio.com/images/ATM-Antibody-2C1-Knockdown-Validated-NB100-309-img0017.jpg "Knockdown Validated: ATM Antibody (2C1) [NB100-309] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:500.")