Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.
Immunohistochemistry: AGTR-2 Antibody [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of ATIIR2 [red] on GFAP+ CSCs [green]. Negative control was a GBM ...read more
Immunohistochemistry-Paraffin: AGTR-2 Antibody [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2, PRR, by cells within GBM, and the ...read more
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of SOX2 [(A), red], PRR [(B), red], & ...read more
Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] - Effects of Ang(1-7) and/or A-779 treatments to the sham & OVX animals for 6 weeks on the expressions of RAS different proteins & osteoclastogenesis modulating ...read more
AGTR2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human AGTR2. The immunogen is located within amino acids 220 - 270 of AGTR2. Amino Acid Squence: LKTNSYGKNRITRDQV
Predicted Species
Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGTR2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in ICC/IF reported in scientific literature (PMID 27200356).
Theoretical MW
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use in Rabbit reported in scientific literature (PMID:35372645).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for AGTR-2 Antibody - BSA Free
AGTR2
AGTR-2
Angiostensin Receptor
angiotensin II receptor, type 2
Angiotensin II type-2 receptor
angiotensin receptor 2
AT2
AT2ATGR2
AT2R
MRX88
MRX88type-2 angiotensin II receptor
Background
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion that acts through at least two types of receptors, AGTR1 and AGTR2. It is an important effector controlling blood pressure and volume in the cardiovascular system and plays a major role in the development of the mammalian kidney and urinary tract. Like AGTR1, AGTR2 is a seven transmembrane G protein-coupled receptor (GPCR), but AGTR2 does not demonstrate most of the classic features of GPCR signaling. AGTR2 is involved in a wide range of activities, including the induction of neurite outgrowth and the inhibition of cellular proliferation, in addition to the known function of mediation of vasoconstriction.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Siljee S, Gower A, Brasch H Et al. Expression of Angiotensin II Receptor 2 in Microcystic Lymphatic Malformation Journal of Vascular Anomalies 2021-08-05 (IF/IHC, Human)
Wickremesekera A, Brasch H, Lee V et al. Cancer stem cell subpopulations in metastatic melanoma to the brain express components of the renin-angiotensin system JCMT 2019-08-27
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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![Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/AGTR-2-Antibody-Western-Blot-NBP1-77368-img0005.jpg "Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.")
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![Immunohistochemistry: AGTR-2 Antibody [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of ATIIR2 [red] on GFAP+ CSCs [green]. Negative control was a GBM tissue section with omission of the primary antibody. Cell nuclei were counterstained with 4', 6'-diamidino-2-phenylindole [blue]. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00051/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AGTR-2-Antibody-Immunohistochemistry-NBP1-77368-img0009.jpg "Immunohistochemistry: AGTR-2 Antibody [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of ATIIR2 [red] on GFAP+ CSCs [green]. Negative control was a GBM tissue section with omission of the primary antibody. Cell nuclei were counterstained with 4', 6'-diamidino-2-phenylindole [blue]. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00051/abstract), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: AGTR-2 Antibody [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2, PRR, by cells within GBM, and the endothelium of the microvessels. Cytoplasmic and nuclear staining of ATIIR2 [brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [blue]. Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00051/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AGTR-2-Antibody-Immunohistochemistry-Paraffin-NBP1-77368-img0010.jpg "Immunohistochemistry-Paraffin: AGTR-2 Antibody [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2, PRR, by cells within GBM, and the endothelium of the microvessels. Cytoplasmic and nuclear staining of ATIIR2 [brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [blue]. Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00051/abstract), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of SOX2 [(A), red], PRR [(B), red], & ATIIR2 [(C), red] on GFAP+ CSCs [(A–C), green] & expression of ACE [(D), green] & ATIIR1 [(E), green] on SOX2+ CSCs [(D,E), red]. Negative control was a GBM tissue section with omission of the primary antibody (F). Cell nuclei were counterstained with 4′, 6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730123), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202415304293.jpg "Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of SOX2 [(A), red], PRR [(B), red], & ATIIR2 [(C), red] on GFAP+ CSCs [(A–C), green] & expression of ACE [(D), green] & ATIIR1 [(E), green] on SOX2+ CSCs [(D,E), red]. Negative control was a GBM tissue section with omission of the primary antibody (F). Cell nuclei were counterstained with 4′, 6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730123), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] - Effects of Ang(1-7) and/or A-779 treatments to the sham & OVX animals for 6 weeks on the expressions of RAS different proteins & osteoclastogenesis modulating factors in the femoral bone heads. (A) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL & OPG. (B) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns & bars represent the mean ± SEM of each group. Statistical significance was considered when *P < 0.05 & **P < 0.01 as compared to Sham group & #P < 0.05 & ##P < 0.01 as compared to OVX group. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-02570-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202416171482.jpg "Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] - Effects of Ang(1-7) and/or A-779 treatments to the sham & OVX animals for 6 weeks on the expressions of RAS different proteins & osteoclastogenesis modulating factors in the femoral bone heads. (A) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL & OPG. (B) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns & bars represent the mean ± SEM of each group. Statistical significance was considered when *P < 0.05 & **P < 0.01 as compared to Sham group & #P < 0.05 & ##P < 0.01 as compared to OVX group. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-02570-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-31020241616180.jpg "Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical-stained sections of moderately differentiated lip cancer samples showing cytoplasmic expression of (pro)renin receptor [(A), brown] within the tumor nests (TNs) & cells within the stroma. Angiotensin-converting enzyme [(B), brown] was expressed on the endothelium of the microvessels within the stroma but not the TNs. Angiotensin II receptor 1 [(C), brown] showed weak to moderate cytoplasmic & nuclear staining by cells within the TNs & cells within the stroma. Strong perinuclear & cytoplasmic staining for angiotensin II receptor 2 [(D), brown] was present in cells within the TNs & the stroma, & skeletal tissue. Nuclei were counterstained with hematoxylin [(A–D), blue]. Original magnification: 200×. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202416175254.jpg "Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical-stained sections of moderately differentiated lip cancer samples showing cytoplasmic expression of (pro)renin receptor [(A), brown] within the tumor nests (TNs) & cells within the stroma. Angiotensin-converting enzyme [(B), brown] was expressed on the endothelium of the microvessels within the stroma but not the TNs. Angiotensin II receptor 1 [(C), brown] showed weak to moderate cytoplasmic & nuclear staining by cells within the TNs & cells within the stroma. Strong perinuclear & cytoplasmic staining for angiotensin II receptor 2 [(D), brown] was present in cells within the TNs & the stroma, & skeletal tissue. Nuclei were counterstained with hematoxylin [(A–D), blue]. Original magnification: 200×. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-31020241616562.jpg "Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated lip squamous cell carcinoma demonstrating expression of (pro)renin receptor (PRR) [(A,B), red] by cells within the tumor nests (TNs) & the cells within the stroma that expressed SALL4 [(A), green]. PRR was also expressed by cells within the stroma that expressed OCT4 [(B), green]. Angiotensin-converting enzyme (ACE) [(C), green] was expressed on the endothelium of the microvessels which expressed SOX2 [(C), red], within the stroma. Staining with ETS-related gene [(G), red] confirmed that ACE [(G), green] was expressed by the endothelium of the microvessels within the stroma. Angiotensin II receptor 1 [(D), green] was demonstrated in cells within the TNs & occasional microvessels that stained positively for SOX2 [(D), red]. Angiotensin II receptor 2 [(E,F), red] was expressed by cells within the TNs, & cells within the stroma that expressed SALL4 [(E), green] & those that expressed OCT4 [(F), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202416163773.jpg "Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated lip squamous cell carcinoma demonstrating expression of (pro)renin receptor (PRR) [(A,B), red] by cells within the tumor nests (TNs) & the cells within the stroma that expressed SALL4 [(A), green]. PRR was also expressed by cells within the stroma that expressed OCT4 [(B), green]. Angiotensin-converting enzyme (ACE) [(C), green] was expressed on the endothelium of the microvessels which expressed SOX2 [(C), red], within the stroma. Staining with ETS-related gene [(G), red] confirmed that ACE [(G), green] was expressed by the endothelium of the microvessels within the stroma. Angiotensin II receptor 1 [(D), green] was demonstrated in cells within the TNs & occasional microvessels that stained positively for SOX2 [(D), red]. Angiotensin II receptor 2 [(E,F), red] was expressed by cells within the TNs, & cells within the stroma that expressed SALL4 [(E), green] & those that expressed OCT4 [(F), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunocytochemistry of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 1 u/ml.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-271120241932624.gif "Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunocytochemistry of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 1 u/ml.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunofluorescence of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-2711202419353826.gif "Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunofluorescence of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 5 ug/ml.")
![Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunohistochemistry of AGTR-2 in rat liver tissue with AGTR-2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-271120242045681.jpg "Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunohistochemistry of AGTR-2 in rat liver tissue with AGTR-2 antibody at 5 ug/mL.")
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