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ARFGEF1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ARFGEF1 Antibody [NBP1-88841] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88841] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ARFGEF1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARFGEF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ARFGEF1 Protein (NBP1-88841PEP)
Publications
Read Publication using NBP1-88841.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ARFGEF1 Antibody

  • ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldinA-inhibited)
  • ARFGEP1P200
  • BIG1p200
  • Brefeldin A-inhibited GEP 1
  • brefeldin A-inhibited guanine nucleotide-exchange protein 1
  • D730028O18Rik
  • DKFZP434L057
  • p200 ARF guanine nucleotide exchange factor
  • p200 ARF-GEP1

Background

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARFGEF1 Antibody (NBP1-88841)(1)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for ARFGEF1 Antibody (NBP1-88841) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on ARFGEF1

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Bioinformatics

Gene Symbol ARFGEF1