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ARFGEF1 Antibody

Images

 
Western Blot: ARFGEF1 Antibody [NB100-55257] - Detection of Human and Mouse BIG1/ARFGEF1 on HeLa whole cell lysates using NB100-55257.
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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ARFGEF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1800 and the C-terminus (residue 1849) of human brefeldin A-inhibited guanine nucleotide-exchange protein 1 using the numbering given in entry NP_006412.2 (GeneID 10565).
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARFGEF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NB100-55257.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARFGEF1 Antibody

  • ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldinA-inhibited)
  • ARFGEP1P200
  • BIG1p200
  • Brefeldin A-inhibited GEP 1
  • brefeldin A-inhibited guanine nucleotide-exchange protein 1
  • D730028O18Rik
  • DKFZP434L057
  • p200 ARF guanine nucleotide exchange factor
  • p200 ARF-GEP1

Background

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARFGEF1 Antibody (NB100-55257)(1)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARFGEF1
Entrez
Uniprot