![Western Blot: ARF1 Antibody (1A9/5) - Azide and BSA Free [NBP3-08340] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).](http://images.novusbio.com/fullsize/ARF1-Antibody-1A9-5-Azide-and-BSA-Free-Western-Blot-NBP3-08340-img0002.jpg "Western Blot: ARF1 Antibody (1A9/5) - Azide and BSA Free [NBP3-08340] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | 1A9/5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07760). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | C-terminal human ARF1 peptide SNQLRNQ (Uniprot: P84077) |
| Localization | Cytoplasmic (Golgi Apparatus) |
| Marker | Golgi Apparatus Marker |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ARF1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for ARF1 Antibody (NBP3-08340)Find related products by research area.
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Arf1: A New Focus In Cancer Drug Therapy ARF1 (ADP-ribosylation factor 1) is a protein in the ARF gene family that is responsible for vesicular trafficking within the cell through its activation of phospholipase D. It is found in the cells golgi apparatus and its main function is intra-Golgi... Read full blog post. |
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| Gene Symbol | ARF1 |
![Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) - Azide and BSA Free [NBP3-08340] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (1A9/5).](http://images.novusbio.com/fullsize/ARF1-Antibody-1A9-5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08340-img0001.jpg "Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) - Azide and BSA Free [NBP3-08340] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (1A9/5).")
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