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Cytohesin-1 Antibody (6G11)

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Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells ...read more
Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human placenta tissue.
Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were ...read more
Immunocytochemistry/ Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were ...read more
Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human tonsil tissue.
Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human breast carcinoma.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
6G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Cytohesin-1 Antibody (6G11) Summary

Immunogen
Purified, His-tagged cytohesin-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYTH1
Purity
PEG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 500
  • Immunohistochemistry-Paraffin 1:200 - 500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1.0 ug/ml
Application Notes
WB: Detects an approx. 48 kDa band representing both cytohesin-1 and cytohesin-2. IP: Only precipitates cytohesin-2.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
PEG purified

Alternate Names for Cytohesin-1 Antibody (6G11)

  • B2-1
  • CYTH1
  • cytoadhesin 1
  • cytohesin 1
  • Cytohesin1
  • Cytohesin-1
  • D17S811ECYTOHESIN-1
  • FLJ34050
  • FLJ41900
  • homolog of secretory protein SEC7
  • PH, SEC7 and coiled-coil domain-containing protein 1
  • pleckstrin homology, Sec7 and coiled-coil domains 1
  • PSCD1
  • SEC7 homolog B2-1
  • SEC7

Background

Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The PSCD1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. PSCD1 protein is 83% homologous to PSCD2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytohesin-1 Antibody (NBP2-22513) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYTH1
Entrez
Uniprot