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Cytohesin 3 Antibody (6D3-1A9)

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Western Blot: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - PSCD3 monoclonal antibody (M01), clone 6D3-1A9 Analysis of PSCD3 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - Analysis of monoclonal antibody to PSCD3 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - Analysis of PSCD3 expression in transfected 293T cell line by PSCD3 monoclonal antibody (M01), clone 6D3-1A9.Lane 1: PSCD3 transfected lysate(46.3 KDa).Lane ...read more
Sandwich ELISA: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - Detection limit for recombinant GST tagged PSCD3 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6D3-1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Cytohesin 3 Antibody (6D3-1A9) Summary

Immunogen
PSCD3 (AAH08191, 1 a.a. ~ 180 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRVVEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK*
Specificity
PSCD3 - pleckstrin homology, Sec7 and coiled-coil domains 3 Please note this antibody was made to Human CYTH3 accession number Q96HS5. This is the 179 amino acid uncharacterized human CYTH3 protein that shares 99% homology with the C-terminal region (221-400) of isoform 1 and 100% homology with the C-terminal region (221-399) of isoform 2 of the characterized human CYTH3 protein (accession number O43739).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CYTH3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytohesin 3 Antibody (6D3-1A9)

  • ARF nucleotide-binding site opener 3
  • ARNO3PH, SEC7 and coiled-coil domain-containing protein 3
  • cytohesin 3
  • General receptor of phosphoinositides 1
  • Grp1
  • GRP1cytohesin-3
  • pleckstrin homology, Sec7 and coiled-coil domains 3
  • Protein ARNO3
  • PSCD3pleckstrin homology, Sec7 and coiled/coil domains 3

Background

This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CYTH3
Entrez
OMIM
Uniprot