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ARF4 Antibody

Images

 
Western Blot: ARF4 Antibody [NBP2-26147] - NIH3T3 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARF4 Antibody [NBP2-26147] - Human Thymus tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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ARF4 Antibody Summary

Immunogen
Peptide with sequence C-SEMTDKLGLQSLRN corresponding to internal region according to NP_001651.1.
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
WB: Approx. 19 kDa band observed in NIH3T3 and HeLa lysates (calculated MW of 20.5 kDa band according to NP_001651.1).
Publications
Read Publication using NBP2-26147.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARF4 Antibody

  • ADP-ribosylation factor 4
  • ARF2ADP-ribosylation factor 2

Background

The ARF4 gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins thatstimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and asactivators of phospholipase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARF4 Antibody (NBP2-26147)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ARF4 Antibody (NBP2-26147). (Showing 1 - 1 of 1 FAQ).

  1. I was looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each that don't cross react with the other protein?
    • The sequence similarity between ARF4 and ARF5 shows very high homology between the two proteins. We have not specifically tested for cross-reactivity between the antibodies, but I think it's very likely that they will cross react.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARF4
Entrez