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ARC/NOL3 Antibody - BSA Free

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Western Blot: ARC/NOL3 Antibody [NB500-257] - Analysis of NB500-257 at 1:1000 dilution. Lane 1: NIH3T3 cells. Lane 2: NIH3T3 cells transfected with NOL3. Lane 3: Recombinant ARC. Lane 4: Recombinant NOL3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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ARC/NOL3 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to residues 125-141 (PELQTLTRPGALRAPRR) of the human Nop30 protein.
Specificity
This is specific for the human Nop30 protein (Gene names - ARC, NOP or NOL3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This antibody has been tested in Western blot, where a band at ~25 kDa is seen. No other applications have been tested. Western blot - Use at 1:500 to 1:1,000 dilution. *The investigator should determine the optimal dilution for each assay. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ARC/NOL3 Overexpression Lysate
Publications
Read Publication using
NB500-257 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reacts with residues 125-141 (PELQTLTRPGALRAPRR) of the human Nop30 protein. Reactivity to other species has not tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARC/NOL3 Antibody - BSA Free

  • Apoptosis repressor with CARD
  • ARC
  • ARCFLJ35304
  • CARD2
  • Muscle-enriched cytoplasmic protein
  • MYC
  • MYP
  • NOL3
  • NOP30
  • NOPnucleolar protein 3
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • Nucleolar protein of 30 kDa

Background

Nop30 (nucleolar protein of 30 kDa) is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. The predominant nucleolar localization of Nop30 points to a potential role in RNA processing in muscle nucleoli. A CARD domain containing protein, designated ARC for apoptosis repressor with CARD is related to Nop30 with a different carboxyl terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARC/NOL3 Antibody (NB500-257)(1)

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Product General Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NOL3
Entrez
OMIM
Uniprot