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Aquaporin-4 Antibody (CL0178)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same ...read more
Western Blot: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Analysis in human cerebral cortex tissue.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human stomach shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL0178
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Aquaporin-4 Antibody (CL0178) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: CPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV
Epitope
FKRRFKEAFS
Predicted Species
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AQP4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Aquaporin-4 Recombinant Protein Antigen (NBP2-52872PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Aquaporin-4 Antibody (CL0178)

  • AQP4
  • AQP-4
  • Aquaporin 4
  • MGC22454
  • MIWC
  • WCH4

Background

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AQP4