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APPL Antibody (4M5X9)

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Western Blot: APPL Antibody (4M5X9) [NBP3-16721] - Western blot analysis of extracts of various cell lines, using APPL1 Rabbit mAb (NBP3-16721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: APPL Antibody (4M5X9) [NBP3-16721] - Western blot analysis of extracts of various cell lines, using APPL1 Rabbit mAb (NBP3-16721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
4M5X9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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APPL Antibody (4M5X9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human APPL1 (Q9UKG1). KKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPD
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
APPL1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for APPL Antibody (4M5X9)

  • Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
  • adaptor protein containing pH domain, PTB domain and leucine zipper motif 1
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1
  • AKT2 interactor
  • APPL
  • APPL1
  • APPLdip13-alpha
  • DCC-interacting protein 13-alpha
  • DIP 13 alpha
  • DIP13A
  • DIP13alpha
  • Dip13-alpha
  • KIAA1428
  • signaling adaptor protein DIP13alpha

Background

APPL is encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol APPL1