![Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616] - Coommassie stained gel showing both free and HDL bound Apolipoprotein B/ApoB eluted from a solid phase resin prepared using anti-Human Apolipoprotein B/ApoB antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-Western-Blot-NB120-7616-img0001.jpg "Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616] - Coommassie stained gel showing both free and HDL bound Apolipoprotein B/ApoB eluted from a solid phase resin prepared using anti-Human Apolipoprotein B/ApoB antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Description | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. |
| Immunogen | Apolipoprotein B/ApoB was isolated from human plasma by density gradient centrifugation followed by HPLC purification. (Uniprot: Q7Z7Q0) |
| Localization | Secreted |
| Specificity | Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | APOB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | This product have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Use in Sandwich-ELISA reported in scientific literature (PMID 26648662) |
|
| Publications |
|
| Storage | Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles. |
| Buffer | 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using NB120-7616 | Applications | Species |
|---|---|---|
| Bakillah A, Tedla F, Ayoub I et al. Plasma Nitration of High-Density and Low-Density Lipoproteins in Chronic Kidney Disease Patients Receiving Kidney Transplants. Mediators of Inflammation. 2015-10-19 [PMID: 26648662] (S-ELISA, Human) | S-ELISA | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Apolipoprotein B/ApoB Antibody (NB120-7616)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616] - Apolipoprotein B/ApoB antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-Immunohistochemistry-Paraffin-NB120-7616-img0002.jpg "Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616] - Apolipoprotein B/ApoB antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.")
![Immunohistochemistry LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDL_R_AF2255_Immunohistochemistry_7149.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDLR_AF2255_Western_Blot_23731.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images/af2255_mouse-ldl-r-affinity-purified-polyclonal-ab-4120241049445.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![N/A Fibroblast Activation Protein alpha/FAP [HRP]](https://images.novusbio.com/images2/DATA_Proprotein_Convertase_9_DPC900_ELISA_786.jpg)
![N/A Fibroblast Activation Protein alpha/FAP [HRP]](https://images.novusbio.com/images2/Proprotein_Convertase_9_DPC900_ELISA_157.jpg)
![Immunohistochemistry Complement Factor H Antibody [Unconjugated]](https://images.novusbio.com/images2/Complement_Factor_H_AF4779_Immunohistochemistry_7182.jpg)
![Immunohistochemistry CD55/DAF Antibody [Unconjugated]](https://images.novusbio.com/images2/CD55_AF2009_Immunohistochemistry_6728.jpg)
![Immunocytochemistry CD55/DAF Antibody [Unconjugated]](https://images.novusbio.com/images2/CD55_AF2009_Immunocytochemistry__Immunofluorescence_23052.jpg)
