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Apolipoprotein A-I/ApoA1 Antibody [Unconjugated]

 (10 Publications)
 
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Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat ...read more
Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A‑I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF3664
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3664-SP
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Apolipoprotein A-I/ApoA1 Antibody [Unconjugated] Summary

Immunogen
Human Plasma ApoA1 and E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647
Specificity
Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOA1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3664 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody [Unconjugated]

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-I/ApoA1 Antibody (AF3664)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(3)
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Human
(4)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF3664 Applications Species
Pierneef L, van Hooij A, de Jong D et al. Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19 iScience 2022-12-26 [PMID: 36590898]
Lorenz Nierves, Jian Guo, Siyuan Chen, Janice Tsui, Anuli C. Uzozie, Jonathan W. Bush et al. Multi-omic profiling of the leukemic microenvironment shows bone marrow interstitial fluid is distinct from peripheral blood plasma Experimental Hematology & Oncology 2022-09-15 [PMID: 36109804]
Erden Eren, Jeannie-Marie Leoutsakos, Juan Troncoso, Constantine G. Lyketsos, Esther S. Oh, Dimitrios Kapogiannis Neuronal-Derived EV Biomarkers Track Cognitive Decline in Alzheimer’s Disease Cells 2022-01-27 [PMID: 35159246]
PJ Yao, E Eren, EJ Goetzl, D Kapogianni Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease Biomedicines, 2021-10-31;9(11):. 2021-10-31 [PMID: 34829816] (Western Blot, Human) Western Blot Human
Kurataka Otsuka, Yusuke Yamamoto, Takahiro Ochiya Uncovering temperature‐dependent extracellular vesicle secretion in breast cancer Journal of Extracellular Vesicles 2020-12-31 [PMID: 33408817]
Nogueras-Ortiz, C J, Mahairaki, V Et al. Astrocyte- and Neuron-Derived Extracellular Vesicles from Alzheimer's Disease Patients Effect Complement-Mediated Neurotoxicity. Cells 7/4/2020 [PMID: 32635578]
U Smole, N Gour, J Phelan, G Hofer, C Köhler, B Kratzer, PA Tauber, X Xiao, N Yao, J Dvorak, L Caraballo, L Puerta, S Rosskopf, J Chakir, E Malle, AP Lane, WF Pickl, S Lajoie, M Wills-Karp Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity Nat. Immunol., 2020-06-22;21(7):756-765. 2020-06-22 [PMID: 32572240] (Immunoprecipitation, Human) Immunoprecipitation Human
H Tadokoro, A Hirayama, R Kudo, M Hasebe, Y Yoshioka, J Matsuzaki, Y Yamamoto, M Sugimoto, T Soga, T Ochiya Adenosine leakage from perforin-burst extracellular vesicles inhibits perforin secretion by cytotoxic T-lymphocytes PLoS ONE, 2020-04-10;15(4):e0231430. 2020-04-10 [PMID: 32275689] (Western Blot, Human) Western Blot Human
Anouk van Hooij, Susan van den Eeden, Renate Richardus, Elisa Tjon Kon Fat, Louis Wilson, Kees L.M.C. Franken et al. Application of new host biomarker profiles in quantitative point-of-care tests facilitates leprosy diagnosis in the field EBioMedicine 2019-09-01 [PMID: 31422044]
Zhou G, Marathe G, Hartiala J, Hazen S, Allayee H, Tang W, McIntyre T Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2). J Biol Chem, 2013-03-18;288(17):11940-8. 2013-03-18 [PMID: 23508960] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol APOA1
Uniprot