Apc10 Antibody (8F1D10) - BSA Free

Images

 
Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining.
Flow Cytometry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red).
Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein. (Expected MW is 47.2 kDa)
Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- ...read more
Immunohistochemistry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of paraffin-embedded colon cancer tissues using ANAPC10 mouse mAb with DAB staining.
ELISA: Apc10 Antibody (8F1D10) [NBP2-61889] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
8F1D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Apc10 Antibody (8F1D10) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Apc10 (AA: 1-185) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANAPC10
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Apc10 Antibody (8F1D10) - BSA Free

  • anaphase promoting complex subunit 10
  • anaphase-promoting complex subunit 10
  • APC10DOC1
  • Cyclosome subunit 10
  • DKFZP564L0562

Background

ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANAPC10