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APC Antibody (KT44)

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
KT44
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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APC Antibody (KT44) Summary

Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Clone KT44 was generated from a recombinant protein containing amino acid residues from the central region mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2. [UniProt# Q61315]
Specificity
This antibody detects full length APC at 300 kDa and proteolytic fragments of APC at lower molecular weights. The antibody detects APC or its fragments in SW480, CaCO2, DLD-1, and Hct116 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
APC
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:250
Theoretical MW
300 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for APC Antibody (KT44)

  • adenomatosis polyposis coli tumor suppressor
  • adenomatosis polyposis coli
  • adenomatous polyposis coli protein
  • adenomatous polyposis coli
  • APC
  • BTPS2
  • Deleted in polyposis 2.5
  • DP2
  • DP2.5DP2
  • DP3
  • FPC
  • GS
  • Min
  • Protein APC

Background

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling (1). APC regulates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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APC Antibody (KT44)
NBP3-23227
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Applications: WB, ICC/IF

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Product General Protocols

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ICC/IF Video Protocol

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Isotype Controls

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Bioinformatics

Gene Symbol APC