APC Antibody (Ali 12-28) - BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF, IHC, IP
Clone
Ali 12-28
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

APC Antibody (Ali 12-28) - BSA Free Summary

Immunogen
N-terminal APC-MBP fusion protein
Localization
Cytoplasmic and Nuclear
Specificity
Ali 12-28 detects both normal and mutant-truncated forms of the APC.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
APC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody has been used routinely for Western blotting on colon cell line HCT116, SW480 and SW837 extracts. Use in Immunohistochemistry-paraffin reported in scientific literature (Awadalla A et al).
Publications
Read Publications using
NB100-667 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for APC Antibody (Ali 12-28) - BSA Free

  • adenomatosis polyposis coli tumor suppressor
  • adenomatosis polyposis coli
  • adenomatous polyposis coli protein
  • adenomatous polyposis coli
  • APC
  • BTPS2
  • Deleted in polyposis 2.5
  • DP2
  • DP2.5DP2
  • DP3
  • FPC
  • GS
  • Min
  • Protein APC

Background

This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APC Antibody (NB100-667)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NB100-667 Applications Species
Awadalla A, Aboelenin H, Hemmaid KZ. Pattern of DNA Hypermethylation of APC, P16, P73, P16, O 6 MMGT, CD99 and CDH1 Genes in Bladder Cancer with Hepatitis C Viral (HCV) Infection International Journal of Cancer Research 2015-08-06 (IHC-P, Human) IHC-P Human
Ranes M, Zaleska M, Sakalas S Et al. Reconstitution of the destruction complex defines roles of AXIN polymers and APC in beta-catenin capture, phosphorylation, and ubiquitylation Molecular cell 2021-08-19 [PMID: 34352208] (WB, Human) WB Human
Abutaily AS et al. Cadherins, catenins APC in pleural malignant mesothelioma. J Pathol 201:355-62. 2003-01-01 [PMID: 14595746]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for APC Antibody (NB100-667) (0)

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Secondary Antibodies

 

Isotype Controls

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The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol APC
Entrez
Uniprot