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Angiostatin Antibody [Unconjugated] - Kringle 1-3

 (8 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF226
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF226-SP
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Angiostatin Antibody [Unconjugated] - Kringle 1-3 Summary

Immunogen
E. coli-derived recombinant human Angiostatin Kringle 1-3
Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
Accession # P00747
Specificity
Detects human Angiostatin in direct ELISAs and Western blots. This antibody will also recognize human plasminogen.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF226 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiostatin Antibody [Unconjugated] - Kringle 1-3

  • Angiostatin
  • HAE4

Background

Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin.  Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810).  It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots.  Plasminogen is also cleaved by MMPs which generate multiple fragments.  Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a downregulation of Bcl2, and an upregulation of thrombospondin-1.  Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Angiostatin Antibody (AF226)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Parasite - Plasmodium falciparum.

We have publications tested in 5 applications: ELISA Development, ICC, Immunogold Electron Microscopy, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
Immunogold Electron Microscopy
(1)
Immunoprecipitation
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Rat
(1)
Parasite - Plasmodium falciparum
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF226 Applications Species
S El Chamy M, MY Icimoto, PMS Melo, A Budu, R Coimbra, ML Gazarini, AK Carmona Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes Malar. J., 2020-08-26;19(1):302. 2020-08-26 [PMID: 32847585] (Western Blot, Human, Parasite - Plasmodium falciparum) Western Blot Human, Parasite - Plasmodium falciparum
Radziwon-Balicka A, Moncada de la Rosa C, Zielnik B, Doroszko A, Jurasz P Temporal and pharmacological characterization of angiostatin release and generation by human platelets: implications for endothelial cell migration. PLoS ONE, 2013-03-15;8(3):e59281. 2013-03-15 [PMID: 23555012] (ICC, Immunogold Electron Microscopy, Western Blot, Human) ICC, Immunogold Electron Microscopy, Western Blot Human
Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid. Gene Ther., 2009-12-01;16(12):1405-15. 2009-12-01 [PMID: 19641530] (Western Blot, Human) Western Blot Human
Yang CT, Lin YC, Lin CL, Lu J, Bu X, Tsai YH, Jia WW Oncolytic herpesvirus with secretable angiostatic proteins in the treatment of human lung cancer cells. Anticancer Res., 2005-05-01;25(3):2049-54. 2005-05-01 [PMID: 16158944] (Immunoprecipitation, Human) Immunoprecipitation Human
Basile DP, Fredrich K, Weihrauch D, Hattan N, Chilian WM Angiostatin and matrix metalloprotease expression following ischemic acute renal failure. Am. J. Physiol. Renal Physiol., 2004-01-06;286(5):F893-902. 2004-01-06 [PMID: 15075185] (Western Blot, Rat) Western Blot Rat
Ponnazhagan S, Mahendra G, Kumar S, Shaw DR, Stockard CR, Grizzle WE, Meleth S Adeno-associated virus 2-mediated antiangiogenic cancer gene therapy: long-term efficacy of a vector encoding angiostatin and endostatin over vectors encoding a single factor. Cancer Res., 2004-03-01;64(5):1781-7. 2004-03-01 [PMID: 14996740] (ELISA Development, Mouse) ELISA Development Mouse
Jurasz P, Alonso D, Castro-Blanco S, Murad F, Radomski MW Generation and role of angiostatin in human platelets. Blood, 2003-07-10;102(9):3217-23. 2003-07-10 [PMID: 12855585] (Western Blot, Human) Western Blot Human
Mulligan-Kehoe MJ, Kleinman HK, Drinane M, Wagner RJ, Wieland C, Powell RJ A truncated plasminogen activator inhibitor-1 protein blocks the availability of heparin-binding vascular endothelial growth factor A isoforms. J. Biol. Chem., 2002-10-14;277(50):49077-89. 2002-10-14 [PMID: 12381729] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol PLG
Uniprot