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Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

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ELISA: Human Angiopoietin-4 ELISA Kit (Chemiluminescence) [NBP2-66730] - Samples were spiked with high concentrations of Human Angiopoietin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Angiopoietin-4 ELISA Kit (Chemiluminescence) [NBP2-66730] - Samples were spiked with high concentrations of Human Angiopoietin-4 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Angiopoietin-4 ELISA Kit (Chemiluminescence) [NBP2-66730] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Human Angiopoietin-4 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.21%
Intra-Assay
CV% < 9.04%
Spike Recovery
89-116%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ANGPT4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

  • AGP4
  • ANG3
  • ANG-3
  • ANG-4
  • ANG4angiopoietin-3
  • angiopoietin 4
  • Angiopoietin-3
  • Angiopoietin-4
  • ANGPT4
  • dJ824F16.2 (angiopoietin 4)
  • MGC138181
  • MGC138183

Background

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  Human Angiopoietin-4 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol ANGPT4