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Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)

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ELISA: Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) [NBP2-62857] - Samples were spiked with high concentrations of Mouse Angiopoietin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) [NBP2-62857] - Samples were spiked with high concentrations of Mouse Angiopoietin-1 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) [NBP2-62857] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Mouse ANG1 in samples. No significant cross-reactivity or interference between Mouse ANG1 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 8.76%
Intra-Assay
CV% < 8.76%
Spike Recovery
94-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ANGPT1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using NBP2-62857.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Mouse
Angiopoietin-1 ELISA ...
NBP2-62857
Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)
Species: Mu
Applications: ELISA

Publications for Angiopoietin-1 ELISA Kit (NBP2-62857)(2)

We have publications tested in 1 application: ELISA.


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Product General Protocols

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FAQs for Angiopoietin-1 ELISA Kit (NBP2-62857). (Showing 1 - 1 of 1 FAQs).

  1. Looking at item #NBP2-62857, what kind of plate reader would I use to read it? OD reader, fluoresnce plate reader
    • The samples will fluoresce, so any plate reader that can detect fluorescence should be fine. You will need to measure the relative light units. See the manual for more information

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Contact Information

Product PDFs

  Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol ANGPT1