How do you dissolve the lyophilized Ab? Water or PBS? The datasheet for NB110-85464 just says "Buffer: Lyophilized" - - in Water? Buffer? Concentration of Buffer? NB110-85467 says Lyophilized from a solution of PBS - again how to dissolve the Ab - which PBS and what concentration?

Name: Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NB110-85464
Rating: 3 (2 reviews)

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The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Description	PLEASE NOTE: 0.02mg sample size is provided in reconstituted format and the 0.1mg size is provided lyophilized. (Please see reconstitution instuctions).
Immunogen	Human recombinant human Angiopoietin-1
Specificity	No cross reactivity with human Angiopoietin-2.
Isotype	IgG2
Clonality	Monoclonal
Host	Mouse
Gene	ANGPT1
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Lyophilized from a 0.2 um filtered solution in PBS. 20 ug size is provided in liquid form, PBS
Preservative	No Preservative
Concentration	LYOPH
Purity	Protein G purified
Reconstitution Instructions	Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

	Bryan Tinsley 08/26/2014
Application:	IF
Species:	Other

	Corey . 07/12/2013
Application:	IHC-Fr
Species:	Human

Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

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Gene Symbol	ANGPT1
Entrez	284(Human)
Uniprot	Q15389()