![Immunohistochemistry: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32000) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32000_mouse-aminoacylase-acy1-mab-15g2-r-298202414304197.jpg "Immunohistochemistry: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32000) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | 15G2-R |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human Aminoacylase/ACY1 aa 55-256 / 408. (Uniprot: Q03154) |
| Localization | Cytoplasm. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACY1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACY1 |
![Western Blot: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Western blot analysis of Aminoacylase/ACY1 on different lysates with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Lane 3: Human kidney tissue lysate (40 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Lane 7: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32000) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32000_mouse-aminoacylase-acy1-mab-15g2-r-3920248453526.jpg "Western Blot: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Western blot analysis of Aminoacylase/ACY1 on different lysates with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Lane 3: Human kidney tissue lysate (40 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Lane 7: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32000) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")