Aminoacylase/ACY1 Antibodies
We offer Aminoacylase/ACY1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Aminoacylase/ACY1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Aminoacylase/ACY1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Aminoacylase/ACY1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Aminoacylase/ACY1 polyclonal antibodies and browse our Aminoacylase/ACY1 monoclonal antibody catalog.
Alternate Names for Aminoacylase/ACY1 Antibodies
anti-Aminoacylase/ACY1 antibody, anti-ACY1 antibody, anti-ACY-1 antibody, anti-ACY1D antibody, anti-acylase antibody, anti-aminoacylase 1 antibody, anti-aminoacylase-1 antibody, anti-EC 3.5.1 antibody, anti-EC 3.5.1.14 antibody, anti-N-acyl-L-amino-acid amidohydrolase antibody
14 Results for "aminoacylase-acy1" in Primary Antibodies
![Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Aminoacylase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Western-Blot-NBP2-00970-img0008.jpg "Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Aminoacylase 1 monoclonal antibody.")
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Human prostate tissue using anti-Aminoacylase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Immunohistochemistry-Paraffin-NBP2-00970-img0007.jpg)
![Flow Cytometry: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Aminoacylase 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Flow-Cytometry-NBP2-00970-img0001.jpg)
![Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aminoacylase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aminoacylase 1.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Western-Blot-NBP2-00970-img0002.jpg)
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Aminoacylase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Immunohistochemistry-Paraffin-NBP2-00970-img0003.jpg)
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Aminoacylase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Immunohistochemistry-Paraffin-NBP2-00970-img0004.jpg)
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Human Kidney tissue using anti-Aminoacylase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Immunohistochemistry-Paraffin-NBP2-00970-img0005.jpg)
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Human liver tissue using anti-Aminoacylase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-1D4-Immunohistochemistry-Paraffin-NBP2-00970-img0006.jpg)
![Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody (M01), clone 4F1-B7.Lane 1: ACY1 transfected lysate(45.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-4F1-B7-Western-Blot-H00000095-M01-img0017.jpg "Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody (M01), clone 4F1-B7.Lane 1: ACY1 transfected lysate(45.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of monoclonal antibody to ACY1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-4F1-B7-Immunocytochemistry-Immunofluorescence-H00000095-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-4F1-B7-Immunohistochemistry-Paraffin-H00000095-M01-img0014.jpg)
![Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - ACY1 monoclonal antibody (M01), clone 4F1-B7. Analysis of ACY1 expression in K-562.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-4F1-B7-Western-Blot-H00000095-M01-img0016.jpg)
![Immunoprecipitation: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 transfected lysate using anti-ACY1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACY1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-4F1-B7-Immunoprecipitation-H00000095-M01-img0015.jpg)
![Western Blot: Aminoacylase/ACY1 Antibody [NBP1-31366] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-Western-Blot-NBP1-31366-img0005.jpg "Western Blot: Aminoacylase/ACY1 Antibody [NBP1-31366] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody [NBP1-31366] - A431, using NBP1-31366 at 1:200 dilution.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31366-img0004.jpg)
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody [NBP1-31366] - Sample: Paraffin-embedded placenta. Aminoacylase/ACY1 antibody dilution: 1:100.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-Immunohistochemistry-Paraffin-NBP1-31366-img0006.jpg)
![Immunohistochemistry: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32000) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32000_mouse-aminoacylase-acy1-mab-15g2-r-298202414304197.jpg "Immunohistochemistry: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32000) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Western blot analysis of Aminoacylase/ACY1 on different lysates with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Lane 3: Human kidney tissue lysate (40 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Lane 7: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32000) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32000_mouse-aminoacylase-acy1-mab-15g2-r-3920248453526.jpg)
![Western Blot: Aminoacylase/ACY1 Antibody [NBP2-14261] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-Western-Blot-NBP2-14261-img0003.jpg "Western Blot: Aminoacylase/ACY1 Antibody [NBP2-14261] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody [NBP2-14261] - Staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.](http://images.novusbio.com/fullsize/Aminoacylase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14261-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Staining of Human ACY1 in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human ACY1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-006-Immunocytochemistry-Immunofluorescence-NBP2-89558-img0001.jpg "Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Staining of Human ACY1 in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human ACY1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Analysis of Human ACY1 expression on HepG2 cells. The cells were treated, stained with purified anti-Human ACY1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-006-Flow-Cytometry-NBP2-89558-img0002.jpg)
![Immunohistochemistry: Aminoacylase/ACY1 Antibody [NBP2-14262] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in subsets of renal tubules.](http://images.novusbio.com/fullsize/Aminoacylase-ACY1-Antibody-Immunohistochemistry-NBP2-14262-img0002.jpg "Immunohistochemistry: Aminoacylase/ACY1 Antibody [NBP2-14262] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in subsets of renal tubules.")