AMICA/JAML Antibody

Images

 
Western Blot: AMICA/JAML Antibody [NBP2-14286] - Analysis in human lung tissue.
Immunohistochemistry-Paraffin: AMICA/JAML Antibody [NBP2-14286] - Immunohistochemical analysis of paraffin embedded mouse kidney tissue using control rabbit IgG (left) and anti-AMICA/JAML antibody (right). Image from ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AMICA/JAML Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: KKTCGNKSSVNSTVLVKNTKKTNPEIKEKPCHFERCEGEKHIYSPIIVREVIEEEEPSEKSEATYMTMHPVWPSLRSDRNNSL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JAML
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Validated for from a verified customer review
  • Western Blot 0.04-0.4 ug/ml
Control Peptide
AMICA/JAML Protein (NBP2-14286PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-14286 in the following applications:

Publications
Read Publications using
NBP2-14286 in the following applications:

Reactivity Notes

Reported reactivity in Mouse kidney tissue from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AMICA/JAML Antibody

  • adhesion molecule, interacts with CXADR antigen 1
  • AMICA
  • AMICA1
  • CREA7-1
  • Gm638
  • JAML

Background

JAML may function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesiveinteractions with CXADR, a protein of the junctional complex of epithelial cell

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMICA/JAML Antibody (NBP2-14286)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Review for AMICA/JAML Antibody (NBP2-14286) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-14286:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin AMICA/JAML NBP2-14286
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 12/15/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedkidney
SpeciesMouse
LotR45096

Comments

CommentsAntibody Storage Conditions:Store at 4C short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles.

Test Sample Information:
Species & Treatments:mouse
Tissue: kidney
Fixative Composition: 4% Paraformaldehyde solution
Fixation Time & Temperature: 24h,4℃
Tissue Processing: paraffin embedding

Antigen Retrieval Details (for IHC-P):
Method & Buffer Used: Citric Acid Antigen Retrieval Buffer
Time & Temperature: >95℃ 20min

Blocking Procedure:
Blocking Solution: 5% Goat serum,0.1%BSA
Time & Temperature: 1h,room temperature

Endogenous Peroxidase Blocking (For IHC-P only)
H2O2 cocktail composition: 3% H2O2
Blocking Time: 10min

Primary Antibody
Dilution: 1:100
Diluent Buffer: 0.1%BSA(PBS)
Time & Temperature: overnight,4℃

Washing Conditions:
Wash Buffer Composition: PBS
Method (Immersion, Rinsing etc.): Immersion
Times/Washing: 5min
Repetitions: 3

Secondary Antibody
Manufacturer and Catalog #: Zhongshan golden bridge ZB-2301
Secondary description:goat anti-rabbit
Dilution: 1:100
Diluent Buffer: PBS
Incubation Time & Temperature: 1h,room temperature

Post-Secondary Washing: 5min X 3 times

Detection Method:
Detection: Zhongshan golden bridge SP-9000
Procedure: according to the instruction book
DAB-Incubation Time (if applicable):3min

Controls:
Negative Control: Control rabbit IgG

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for AMICA/JAML Antibody (NBP2-14286) (0)

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Recent Reviews

5
5
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2
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1
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Verified Customer
12/15/2016
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol JAML
Entrez