alpha-Smooth Muscle Actin Antibody (4A4) - BSA Free

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 10.

Publications using NBP2-22120	Applications	Species
Cheng S, Zou Y, Zhang M et al. Single-cell RNA sequencing reveals the heterogeneity and intercellular communication of hepatic stellate cells and macrophages during liver fibrosis MedComm (2020) 2023-09-17 [PMID: 37724132]
Peng L, Jin X, He Q et al. Remodelling landscape of tissue-engineered bladder with porcine small intestine submucosa using single-cell RNA sequencing Cell proliferation 2022-09-30 [PMID: 36177893] (IF/IHC, Mouse)	IF/IHC	Mouse
Chun-Shan Liu, Inmaculada Rioja, Ali Bakr, Marlon R Veldwijk, Elena Sperk, Carsten Herskind, Dieter Weichenhan, Rab K Prinjha, Christoph Plass, Peter Schmezer, Odilia Popanda Selective inhibitors of bromodomain BD1 and BD2 of BET proteins modulate radiation-induced profibrotic fibroblast responses. International journal of cancer 2022-07-15 [PMID: 35239184]
Kamothi DJ, Kant V, Jangir BL et al. Novel preparation of bilirubin-encapsulated pluronic F-127 nanoparticles as a potential biomaterial for wound healing European journal of pharmacology 2022-03-15 [PMID: 35151648] (IHC-P, Rat)	IHC-P	Rat
Kant V, Jangir Bl, Sharma M Et Al. Topical application of quercetin improves wound repair and regeneration in diabetic rats Immunopharmacology and immunotoxicology 2021-07-19 [PMID: 34278923]
Choudhary A, Kant V, Jangir BL, Joshi VG Quercetin loaded chitosan tripolyphosphate nanoparticles accelerated cutaneous wound healing in Wistar rats Eur. J. Pharmacol. 2020-05-11 [PMID: 32407724] (IF/IHC, Rat)	IF/IHC	Rat
Zhou P, Zhang X, Guo M et al. Ginsenoside Rb1 ameliorates CKD-associated vascular calcification by inhibiting the Wnt/beta-catenin pathway J. Cell. Mol. Med. 2019-08-19 [PMID: 31423730] (WB, Rat)	WB	Rat
Chakrabarti J, Holokai L, Syu L et al. Hedgehog signaling induces PD-L1 expression and tumor cell proliferation in gastric cancer. Oncotarget 2018-12-21 [PMID: 30647844]
Jensen T, Wanczyk H, Sharma I et al. Polyurethane scaffolds seeded with autologous cells can regenerate long esophageal gaps: An esophageal atresia treatment model. J Pediatr Surg. 2018-10-22 [PMID: 30429066] (ICC/IF) Details: Citation using the Azide and BSA Free form of this antibody.	ICC/IF
Guo M, Guo G, Xiao J et al. Ginsenoside Rg3 stereoisomers differentially inhibit vascular smooth muscle cell proliferation and migration in diabetic atherosclerosis. J Cell Mol Med 2018-06-01 [PMID: 29566305] (Mouse)		Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Guo Mengqi	IHC-Fr	Mouse	01/23/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse aortic root Species Mouse Lot Lot100101

Array NBP2-22120

• alpha-Smooth Muscle Actin Antibodies
• alpha-Smooth Muscle Actin Antibody Pair
• alpha-Smooth Muscle Actin ELISA Kits
• alpha-Smooth Muscle Actin Lysates
• alpha-Smooth Muscle Actin Proteins