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alpha-Fetoprotein/AFP Antibody (OTI2A9) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI2A9
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70103AF350
Alexa Fluor 405
NBP2-70103AF405
Alexa Fluor 488
NBP2-70103AF488
Alexa Fluor 532
NBP2-70103AF532
Alexa Fluor 594
NBP2-70103AF594
Alexa Fluor 647
NBP2-70103AF647
Alexa Fluor 700
NBP2-70103AF700
Alexa Fluor 750
NBP2-70103AF750
Allophycocyanin
NBP2-70103APC
Biotin
NBP2-70103B
DyLight 350
NBP2-70103UV
DyLight 405
NBP2-70103V
DyLight 488
NBP2-70103G
DyLight 550
NBP2-70103R
DyLight 594
NBP2-70103DL594
DyLight 650
NBP2-70103C
DyLight 680
NBP2-70103FR
DyLight 755
NBP2-70103IR
FITC
NBP2-70103F
HRP
NBP2-70103H
Janelia Fluor 525
NBP2-70103JF525
Janelia Fluor 549
NBP2-70103JF549
Janelia Fluor 585
NBP2-70103JF585
Janelia Fluor 635
NBP2-70103JF635
Janelia Fluor 646
NBP2-70103JF646
Janelia Fluor 669
NBP2-70103JF669
PE
NBP2-70103PE
PerCP
NBP2-70103PCP
Unconjugated
NBP2-03084
mFluor Violet 450 SE
NBP2-70103MFV450
mFluor Violet 500 SE
NBP2-70103MFV500
mFluor Violet 610 SE
NBP2-70103MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-03084
Azide and BSA Free
NBP2-70103
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alpha-Fetoprotein/AFP Antibody (OTI2A9) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for alpha-Fetoprotein/AFP Antibody (OTI2A9) [CoraFluor™ 1]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AFP