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alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)Species Glossary
Applications ELISA, Flow, IHC, CyTOF-ready, KO
Clone
C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34577AF350
Alexa Fluor 405
NBP2-34577AF405
Alexa Fluor 488
NBP2-34577AF488
Alexa Fluor 532
NBP2-34577AF532
Alexa Fluor 594
NBP2-34577AF594
Alexa Fluor 647
NBP2-34577AF647
Alexa Fluor 700
NBP2-34577AF700
Alexa Fluor 750
NBP2-34577AF750
Allophycocyanin
NBP2-34577APC
Biotin
NBP2-34577B
CoraFluor 1
NBP2-34577CL1
DyLight 350
NBP2-34577UV
DyLight 405
NBP2-34577V
DyLight 488
NBP2-34577G
DyLight 550
NBP2-34577R
DyLight 594
NBP2-34577DL594
DyLight 650
NBP2-34577C
DyLight 680
NBP2-34577FR
DyLight 755
NBP2-34577IR
FITC
NBP2-34577F
HRP
NBP2-34577H
Janelia Fluor 549
NBP2-34577JF549
Janelia Fluor 585
NBP2-34577JF585
Janelia Fluor 635
NBP2-34577JF635
Janelia Fluor 646
NBP2-34577JF646
Janelia Fluor 669
NBP2-34577JF669
PE
NBP2-34577PE
PerCP
NBP2-34577PCP
Unconjugated
NBP2-32914-0.1mg
mFluor Violet 450 SE
NBP2-34577MFV450
mFluor Violet 500 SE
NBP2-34577MFV500
mFluor Violet 610 SE
NBP2-34577MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32914-0.1mg
IHC-Prediluted
NBP2-45009
Azide and BSA Free
NBP2-34577-0.1mg
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alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 525] Summary

Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771)
Localization
Cytoplasmic
Marker
Hepatocellular/Germ Cell Tumor Marker
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Sandwich ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Bovine, Mouse or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 525]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AFP