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Human Aldolase A ELISA Kit (Colorimetric)

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ELISA: Human Aldolase A ELISA Kit (Colorimetric) [NBP3-18760] - Samples were spiked with high concentrations of Human Aldolase A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Aldolase A ELISA Kit (Colorimetric) [NBP3-18760] - Samples were spiked with high concentrations of Human Aldolase A and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human Aldolase A ELISA Kit (Colorimetric) [NBP3-18760] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human Aldolase A ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Aldolase A in samples. No significant cross-reactivity or interference between Human Aldolase A and analogues was observed.
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 4.80
Intra-Assay
%CV < 6.12
Spike Recovery
91-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ALDOA

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Aldolase A ELISA Kit (Colorimetric)

  • ALDA
  • aldolase A, fructose-bisphosphate
  • EC 4.1.2.13
  • fructose-1,6-bisphosphate triosephosphate-lyase
  • fructose-bisphosphate aldolase A
  • GSD12
  • Lung cancer antigen NY-LU-1
  • MGC10942
  • MGC17716
  • MGC17767
  • Muscle-type aldolase

Background

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ALDOA