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Aggrecan Antibody (6-B-4)

 (3 Publications)
 (1 Review)
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Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524] - Aggrecan on sheep intervertebral disc. Image from verified customer review.
Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524] - Aggrecan on sheep intervertebral disc. Image from verified customer review.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB110-6524
    • Availability
      Product Discontinued

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Aggrecan Antibody (6-B-4) Summary

Immunogen
Synthetic peptide: EPEEPFTFAPEI.
Marker
Chondrocyte Marker
Specificity
Recognises the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobular domain (IGD) of human and bovine aggrecan.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACAN
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100
Application Notes
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB110-6524 in the following applications:
Publications
Read Publications using
NB110-6524 in the following applications:

Reactivity Notes

Cross-reacts with Human and Cow. Sheep data from customer review. Mouse reactivity reported in scientific literature (PMID: 31364704).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tissue culture supernatant
Preservative
0.002% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Aggrecan Antibody (6-B-4)

  • ACAN
  • AGC1
  • AGC1SEDK
  • aggrecan core protein
  • Aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16
  • MSK16AGCAN
  • SEDK

Background

This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Aggrecan Antibody
(6-B-4)
NB110-6524
Aggrecan Antibody (6-B-4)
Species: Hu, Mu, Bv
Applications: WB, ELISA, IHC

Publications for Aggrecan Antibody (NB110-6524)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF/IHC, WB.


Filter By Application
IF/IHC
(1)
WB
(1)
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Human
(1)
Mouse
(1)
All Species

Review for Aggrecan Antibody (NB110-6524) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB110-6524:
Filter by Applications
IHC-P
(1)
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Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Aggrecan NB110-6524
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Other 08/04/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSheep intervertebral disc
SpeciesOther

Product General Protocols

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Video Protocols

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FAQs for Aggrecan Antibody (NB110-6524) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Aggrecan Antibody (NB110-6524)

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Blogs on Aggrecan.

Check out the latest blog posts on Aggrecan.
Aches & Pains: Aggrecan in Joint Disease and Osteoarthritis
Aggrecan is essential for the normal function of articular cartilage and intervertebral discs. Aggrecan provides the ability for the tissues to withstand compressive loading. This property is dependent on both the high charge density endowed by its nu...  Read full blog post.
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Recent Reviews

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1
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Immunohistochemistry-Paraffin Aggrecan NB110-6524
Enlarge
 Verified Customer
08/04/2014
Application: IHC-P
Species: Other
read this review

Bioinformatics

Gene Symbol ACAN
Entrez