![Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196] - Western blotting analysis was performed on whole cell extracts (HOT lysis buffer) of MCF-7, HeLa, and Jurkat cell lines, mixed and heated (100C, 5 min) with reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody AGR3.4 (1 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.AGR2/AGR3 molecules were detected at ~ 14-18 kDa in MCF-7 cell line.](http://images.novusbio.com/fullsize/antibody/nbp2-62196_mouse-monoclonal-ag-3-agr3-antibody-agr3-4-western-blot-11122024114746..jpg "Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196] - Western blotting analysis was performed on whole cell extracts (HOT lysis buffer) of MCF-7, HeLa, and Jurkat cell lines, mixed and heated (100C, 5 min) with reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody AGR3.4 (1 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.AGR2/AGR3 molecules were detected at ~ 14-18 kDa in MCF-7 cell line.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | AGR3.4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Purified human AGR3 protein |
| Specificity | The antibody DA-7 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AGR3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS and 0.2% (w/v) high grade BSA (protease free) |
| Preservative | 0.9% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for AG-3/AGR3 Antibody (NBP2-62196)Find related products by research area.
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| Gene Symbol | AGR3 |
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