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AG-3/AGR3 Antibody (774855)

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Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AG-3 Monoclonal Antibody (Catalog # MAB6307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AG-3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AG-3 Monoclonal Antibody (Catalog # MAB6307) followed by HRP-conjugated Anti-Mouse IgG ...read more
AG-3 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human AG-3 Monoclonal Antibody (Catalog # MAB6307) at 15 µg/mL overnight at 4 °C. Before incubation with the primary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
774855
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6307-SP
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AG-3/AGR3 Antibody (774855) Summary

Immunogen
E. coli-derived recombinant human AG-3
Ile22-Leu166
Accession # Q8TD06
Specificity
Detects human AG-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human AG-2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AGR3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AG-3/AGR3 Antibody (774855)

  • AG3
  • AG-3
  • AGR3
  • anterior gradient homolog 3 (Xenopus laevis)
  • BCMP11
  • BCMP11AG3
  • Breast cancer membrane protein 11
  • hAG-3member 18
  • PDIA18

Background

AG-3 (Anterior gradient protein 3; also known as AGR3 and BCMP11) is a 17 kDa (predicted) member of the AGR family of proteins. It is expressed by intestinal epithelial and breast cancer cells. AG-3 is presumably secreted and binds to both alpha -dystroglycan and C4.4a/LYPD3. Mature human AG-3 is 145 amino acids (aa) in length (aa 22‑166). Over aa 22-166, human AG-3 shares 92% amino acid sequence identity with mouse AG-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AG-3/AGR3 Antibody
(774855)
MAB6307
AG-3/AGR3 Antibody (774855)
Species: Hu
Applications: WB, IHC

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Bioinformatics

Gene Symbol AGR3
Uniprot