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Adenylate Kinase 1 Antibody

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Immunohistochemistry: Adenylate Kinase 1 Antibody [NBP3-35229] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using Adenylate Kinase 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen ...read more
Western Blot: Adenylate Kinase 1 Antibody [NBP3-35229] - Western blot analysis of various lysates using Adenylate Kinase 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody [NBP3-35229] - Immunofluorescence analysis of HeLa cells using Adenylate Kinase 1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG ...read more
Immunohistochemistry: Adenylate Kinase 1 Antibody [NBP3-35229] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Adenylate Kinase 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Adenylate Kinase 1 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human Adenylate Kinase 1 (NP_000467.1).

Sequence:
MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AK1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Adenylate Kinase 1 Antibody

  • Adenylate Kinase 1
  • adenylate kinase isoenzyme 1
  • AK 1
  • AK1
  • ATP-AMP transphosphorylase 1
  • EC 2.7.4
  • EC 2.7.4.3
  • EC 2.7.4.6
  • Myokinase

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AK1