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ACTR3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody [NBP3-23369] - Immunocytochemical labeling of actin-related protein 2/3 complex subunit 1B in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated ...read more
Western Blot: ACTR3 Antibody [NBP3-23369] - Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ACTR3 Antibody Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
ACTR3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human ACTR3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast ACTR3 proteins. [UniProt# P61158]
Specificity
The antibody detects a 47 kDa doublet corresponding to the molecular mass of ACTR3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTR3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Alternate Names for ACTR3 Antibody

  • actin-related protein 3
  • ACTR3
  • ARP3 (actin-related protein 3, yeast) homolog
  • ARP3 actin-related protein 3 homolog (yeast)
  • ARP3
  • ARP3Actin-like protein 3

Background

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACTR3