![Western Blot: ACTR3 Antibody [NBP2-97009] - Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/ACTR3-Antibody-Western-Blot-NBP2-97009-img0005.jpg "Western Blot: ACTR3 Antibody [NBP2-97009] - Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Human ACTR3 (Accession#: NP_005712.1; Ala2-Ser418). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ACTR3 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ACTR3 Antibody (NBP2-97009)Find related products by research area.
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| Gene Symbol | ACTR3 |
![Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP2-97009] - Immunofluorescence staining of ACTR3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ACTR3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/ACTR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97009-img0001.jpg "Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP2-97009] - Immunofluorescence staining of ACTR3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ACTR3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunoprecipitation: ACTR3 Antibody [NBP2-97009] - ACTR3 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-ACTR3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ACTR3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa](http://images.novusbio.com/fullsize/ACTR3-Antibody-Immunoprecipitation-NBP2-97009-img0004.jpg "Immunoprecipitation: ACTR3 Antibody [NBP2-97009] - ACTR3 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-ACTR3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ACTR3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa")
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