Home » ACE-2 » ACE-2 Antibodies » ACE-2 Antibody (171608) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

ACE-2 Antibody (171608) [Unconjugated]

 (7 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9331) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9331) followed by HRP-conjugated ...read more
ACE‑2 was detected in immersion fixed frozen sections of rat kidney using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 1 µg/mL overnight at 4 °C. Before incubation ...read more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 10 µg/mL for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity Hu, Rt, HaSpecies Glossary
Applications WB, IHC, IP
Clone
171608
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9331-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

ACE-2 Antibody (171608) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740 (predicted)
Accession # Q9BYF1
Specificity
Detects recombinant human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ACE-1 or recombinant mouse ACE-1 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACE2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB9331 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (171608) [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ACE/CD143 Antibody
[Unconjugated]
AF1513
Immunohistochemistry ACE/CD143 Antibody [Unconjugated]
Western Blot ACE/CD143 Antibody [Unconjugated]
Simple Western ACE/CD143 Antibody [Unconjugated]
(1 Review)
(7 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
Angiogenin [HRP]
DAN00
N/A Angiogenin [HRP]
N/A Angiogenin [HRP]
(23 Publications)
Species: Hu
Applications: ELISA
Mas Antibody - BSA
Free
NBP1-78444
Simple Western Mas Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Mas Antibody - BSA Free
Immunohistochemistry Mas Antibody - BSA Free
(2 Reviews)
(21 Publications)
Species: Hu, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Renin Antibody
(411507) - Propeptide
MAB4447
Immunohistochemistry Renin Antibody (411507) - Propeptide
(6 Publications)
Species: Hu
Applications: IHC, WB
AGTR-1 Antibody -
BSA Free
NBP1-77078
Western Blot AGTR-1 Antibody - BSA Free
Immunohistochemistry AGTR-1 Antibody - BSA Free
Immunohistochemistry AGTR-1 Antibody - BSA Free
(10 Publications)
Species: Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Neprilysin/CD10
Antibody [Unconjugate
AF1126
Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]
Western Blot Neprilysin/CD10 Antibody [Unconjugated]
Western Blot Neprilysin/CD10 Antibody [Unconjugated]
(22 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
Acetyl-coenzyme A
transporter 1 Antibod ...
H00009197-M07
Western Blot Acetyl-coenzyme A transporter 1 Antibody (3A4)
Western Blot Acetyl-coenzyme A transporter 1 Antibody (3A4)
Western Blot Acetyl-coenzyme A transporter 1 Antibody (3A4)
(2 Publications)
Species: Ha, Hu, Mu, Rt
Applications: ELISA, ICC/IF, KD, WB
ATM Antibody (2C1)
NB100-309
Knockdown Validated ATM Antibody (2C1)
Knockdown Validated ATM Antibody (2C1)
Immunoprecipitation ATM Antibody (2C1)
(3 Reviews)
(131 Publications)
Species: Hu, Pm, Mu, Rt
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD, PAGE, Single-Cell Western, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(256 Publications)
Species: Hu
Applications: ELISA
Collectrin/TMEM27
Antibody [Unconjugate ...
AF4965
Western Blot Collectrin/TMEM27 Antibody [Unconjugated]
Immunohistochemistry Collectrin/TMEM27 Antibody [Unconjugated]
Simple Western Collectrin/TMEM27 Antibody [Unconjugated]
(1 Publication)
Species: Mu
Applications: IHC, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA

Acetylcholinesterase/A
NB100-1519
Flow Cytometry Acetylcholinesterase/ACHE Antibody
Western Blot Acetylcholinesterase/ACHE Antibody
Immunocytochemistry/ Immunofluorescence Acetylcholinesterase/ACHE Antibody
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(663 Publications)
Species: Mu
Applications: ELISA

Publications for ACE-2 Antibody (MAB9331)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 3 applications: Blocking/Neutralizing, Flow Cytometry, Western Blot.


Filter By Application
Blocking/Neutralizing
(1)
Flow Cytometry
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Hamster
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB9331 Applications Species
Bejoy, J;Williams, CI;Cole, HJ;Manzoor, S;Davoodi, P;Battaile, JI;Kaushik, A;Nikolaienko, SI;Brelidze, TI;Gychka, SG;Suzuki, YJ; Effects of spike proteins on angiotensin converting enzyme 2 (ACE2) Archives of biochemistry and biophysics 2023-09-26 [PMID: 37769892] (Blocking/Neutralizing, Human) Blocking/Neutralizing Human
Lauren Grimm, Chinyere Onyeukwu, Grace Kenny, Danielle M. Parent, Jia Fu, Shaurya Dhingra et al. Immune Dysregulation in Acute SARS-CoV-2 Infection Pathogens and Immunity 2/20/2023 [PMID: 36865568]
Cecilia Lindskog, Loren Méar, Johan Virhammar, David Fällmar, Eva Kumlien, Göran Hesselager et al. Protein Expression Profile of ACE2 in the Normal and COVID-19-Affected Human Brain Journal of Proteome Research 9/2/2022 [PMID: 35901083]
T Yamaguchi, M Hoshizaki, T Minato, S Nirasawa, MN Asaka, M Niiyama, M Imai, A Uda, JF Chan, S Takahashi, J An, A Saku, R Nukiwa, D Utsumi, M Kiso, A Yasuhara, VK Poon, CC Chan, Y Fujino, S Motoyama, S Nagata, JM Penninger, H Kamada, KY Yuen, W Kamitani, K Maeda, Y Kawaoka, Y Yasutomi, Y Imai, K Kuba ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury Nature Communications, 2021-11-23;12(1):6791. 2021-11-23 [PMID: 34815389] (Western Blot, Hamster) Western Blot Hamster
MN Asaka, D Utsumi, H Kamada, S Nagata, Y Nakachi, T Yamaguchi, Y Kawaoka, K Kuba, Y Yasutomi Highly susceptible SARS-CoV-2 model in CAG promoter-driven hACE2 transgenic mice JCI Insight, 2021-10-08;0(0):. 2021-10-08 [PMID: 34463644] (Western Blot, Mouse) Western Blot Mouse
Han DP, Penn-Nicholson A, Cho MW Identification of critical determinants on ACE2 for SARS-CoV entry and development of a potent entry inhibitor. Virology, 2006-02-28;350(1):15-25. 2006-02-28 [PMID: 16510163] (Flow Cytometry, Human) Flow Cytometry Human
Jiang Y, Xu J, Zhou C, Wu Z, Zhong S, Liu J, Luo W, Chen T, Qin Q, Deng P Characterization of cytokine/chemokine profiles of severe acute respiratory syndrome. Am. J. Respir. Crit. Care Med., 2005-01-18;171(8):850-7. 2005-01-18 [PMID: 15657466] (Flow Cytometry, Human) Flow Cytometry Human

Reviews for ACE-2 Antibody (MAB9331) (0)

There are no reviews for ACE-2 Antibody (MAB9331). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ACE-2 Antibody (MAB9331) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ACE-2 Products

Blogs on ACE-2.

Check out the latest blog posts on ACE-2.
COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.
COVID-19 and the Cardiovascular System: Observed complications and potential mechanisms
By Victoria OsinskiThe outbreak of COVID-19 resulting from the transmission of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in many cases of illness typically manifesting in mi...  Read full blog post.

  Read full blog post.

  Read full blog post.
Tiny Antibodies (VHHs) from Llama Neutralize Respiratory Coronaviruses
By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.
Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ACE-2 Antibody (171608) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol ACE2
Uniprot