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53BP1 Antibody (1285C) [mFluor Violet 450 SE]

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53BP1 Antibody (1285C) [mFluor Violet 450 SE] [NBP2-54753MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.
53BP1 Antibody (1285C) [mFluor Violet 450 SE] [NBP2-54753MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
1285C
Clonality
Monoclonal
Host
Rabbit
Conjugate
mFluor Violet 450 SE

Order Details

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View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54753AF350
Alexa Fluor 405
NBP2-54753AF405
Alexa Fluor 488
NBP2-54753AF488
Alexa Fluor 532
NBP2-54753AF532
Alexa Fluor 594
NBP2-54753AF594
Alexa Fluor 647
NBP2-54753AF647
Alexa Fluor 700
NBP2-54753AF700
Alexa Fluor 750
NBP2-54753AF750
Allophycocyanin
NBP2-54753APC
Biotin
NBP2-54753B
CoraFluor 1
NBP2-54753CL1
DyLight 350
NBP2-54753UV
DyLight 405
NBP2-54753V
DyLight 488
NBP2-54753G
DyLight 550
NBP2-54753R
DyLight 594
NBP2-54753DL594
DyLight 650
NBP2-54753C
DyLight 680
NBP2-54753FR
DyLight 755
NBP2-54753IR
FITC
NBP2-54753F
HRP
NBP2-54753H
Janelia Fluor 525
NBP2-54753JF525
Janelia Fluor 549
NBP2-54753JF549
Janelia Fluor 646
NBP2-54753JF646
Janelia Fluor 669
NBP2-54753JF669
PE
NBP2-54753PE
PerCP
NBP2-54753PCP
Unconjugated
NBP2-54753
mFluor Violet 500 SE
NBP2-54753MFV500
mFluor Violet 610 SE
NBP2-54753MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-54753
BSA Free
NBP2-54753SS
BSA Free
NBP2-54753
Azide and BSA Free
NBP2-80550
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53BP1 Antibody (1285C) [mFluor Violet 450 SE] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
53BP1 Antibody (1285C) was made to a synthetic peptide made to the C-terminal portion of human 53BP1 protein (between amino acids 1900-1972) [UniProt Q12888]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TP53BP1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Feline (100%), Gibbon (100%), Gorilla (100%), Hamster (100%), Marmoset (100%), Orangutan (100%), Panda (100%), Porcine (100%), Rabbit (100%), Sheep (100%), Bat (99%), Elephant (99%), Rat (99%)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for 53BP1 Antibody (1285C) [mFluor Violet 450 SE]

  • 53BP1
  • p202
  • p53-binding protein 1
  • p53bp1
  • TDRD30
  • TP53-Binding Protein 1
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TP53BP1