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53BP1 Antibody (1285C) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
1285C
Clonality
Monoclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

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View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54753AF350
Alexa Fluor 405
NBP2-54753AF405
Alexa Fluor 488
NBP2-54753AF488
Alexa Fluor 532
NBP2-54753AF532
Alexa Fluor 594
NBP2-54753AF594
Alexa Fluor 647
NBP2-54753AF647
Alexa Fluor 700
NBP2-54753AF700
Alexa Fluor 750
NBP2-54753AF750
Allophycocyanin
NBP2-54753APC
Biotin
NBP2-54753B
CoraFluor 1
NBP2-54753CL1
DyLight 350
NBP2-54753UV
DyLight 405
NBP2-54753V
DyLight 488
NBP2-54753G
DyLight 550
NBP2-54753R
DyLight 594
NBP2-54753DL594
DyLight 680
NBP2-54753FR
DyLight 755
NBP2-54753IR
FITC
NBP2-54753F
HRP
NBP2-54753H
Janelia Fluor 525
NBP2-54753JF525
Janelia Fluor 549
NBP2-54753JF549
Janelia Fluor 646
NBP2-54753JF646
Janelia Fluor 669
NBP2-54753JF669
PE
NBP2-54753PE
PerCP
NBP2-54753PCP
Unconjugated
NBP2-54753
mFluor Violet 450 SE
NBP2-54753MFV450
mFluor Violet 500 SE
NBP2-54753MFV500
mFluor Violet 610 SE
NBP2-54753MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-54753
BSA Free
NBP2-54753SS
BSA Free
NBP2-54753
Azide and BSA Free
NBP2-80550
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53BP1 Antibody (1285C) [DyLight 650] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
53BP1 Antibody (1285C) was made to a synthetic peptide made to the C-terminal portion of human 53BP1 protein (between amino acids 1900-1972) [UniProt Q12888]
Predicted Species
Rat (93%), Porcine (100%), Bovine (100%), Feline (100%), Canine (100%), Primate (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TP53BP1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Feline (100%), Gibbon (100%), Gorilla (100%), Hamster (100%), Marmoset (100%), Orangutan (100%), Panda (100%), Porcine (100%), Rabbit (100%), Sheep (100%), Bat (99%), Elephant (99%), Rat (99%)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for 53BP1 Antibody (1285C) [DyLight 650]

  • 53BP1
  • p202
  • p53-binding protein 1
  • p53bp1
  • TDRD30
  • TP53-Binding Protein 1
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TP53BP1