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15-PGDH/HPGD Antibody (OTI2C10)

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Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.
Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.
Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-15-PGDH antibody, and ...read more
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.
Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI2C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70933AF350
Alexa Fluor 405
NBP2-70933AF405
Alexa Fluor 488
NBP2-70933AF488
Alexa Fluor 532
NBP2-70933AF532
Alexa Fluor 594
NBP2-70933AF594
Alexa Fluor 647
NBP2-70933AF647
Alexa Fluor 700
NBP2-70933AF700
Alexa Fluor 750
NBP2-70933AF750
Allophycocyanin
NBP2-70933APC
Biotin
NBP2-70933B
CoraFluor 1
NBP2-70933CL1
DyLight 350
NBP2-70933UV
DyLight 405
NBP2-70933V
DyLight 488
NBP2-70933G
DyLight 550
NBP2-70933R
DyLight 594
NBP2-70933DL594
DyLight 650
NBP2-70933C
DyLight 680
NBP2-70933FR
DyLight 755
NBP2-70933IR
FITC
NBP2-70933F
HRP
NBP2-70933H
Janelia Fluor 525
NBP2-70933JF525
Janelia Fluor 549
NBP2-70933JF549
Janelia Fluor 646
NBP2-70933JF646
Janelia Fluor 669
NBP2-70933JF669
PE
NBP2-70933PE
PerCP
NBP2-70933PCP
mFluor Violet 450 SE
NBP2-70933MFV450
mFluor Violet 500 SE
NBP2-70933MFV500
mFluor Violet 610 SE
NBP2-70933MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70933
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15-PGDH/HPGD Antibody (OTI2C10) Summary

Immunogen
Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HPGD
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 15-PGDH/HPGD Antibody (OTI2C10)

  • 15-hydroxyprostaglandin dehydrogenase [NAD+]
  • 15PGDH
  • 15-PGDH
  • EC 1.1.1
  • EC 1.1.1.141
  • HPGD
  • hydroxyprostaglandin dehydrogenase 15-(NAD)
  • NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
  • PGDH1
  • PGDH1PGDH
  • Prostaglandin dehydrogenase 1
  • SDR36C1
  • short chain dehydrogenase/reductase family 36C, member 1

Background

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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15-PGDH/HPGD
Antibody (OTI2C10)
NBP2-00490
15-PGDH/HPGD Antibody (OTI2C10)
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HPGD
Entrez