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![Western Blot: HSPB8/HSP22 Antibody [NBP1-84945] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPB8 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Western-Blot-NBP1-84945-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSPB8/HSP22 Antibody [NBP1-84945] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPB8 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-84945] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84945-img0007.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NBP1-84945-img0013.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NBP1-84945-img0010.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NBP1-84945-img0011.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NBP1-84945-img0012.jpg)
![Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Staining of Human Muscle lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Western-Blot-NB100-1123-img0005.jpg "Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Staining of Human Muscle lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123] - Staining of paraffin embedded Human Tonsil. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NB100-1123-img0007.jpg)
![Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Analysis of H11/HSP22 in Human Muscle lysate using NB100-1123 at 1 ug/mL.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Western-Blot-NB100-1123-img0004.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NB100-1123-img0006.jpg)
![Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp22-Antibody-1E3-Western-Blot-NBP2-02576-img0005.jpg "Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Hsp22 Antibody (1E3) [NBP2-02576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp22.](http://images.novusbio.com/fullsize/Hsp22-Antibody-1E3-Immunocytochemistry-Immunofluorescence-NBP2-02576-img0004.jpg)
![Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Hsp22 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Hsp22-Antibody-1E3-Flow-Cytometry-NBP2-02576-img0002.jpg)
![Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hsp22.](http://images.novusbio.com/fullsize/Hsp22-Antibody-1E3-Western-Blot-NBP2-02576-img0003.jpg)
![Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - Analysis of Jurkat cells, using anti-Hsp22 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Hsp22-Antibody-1E3-Flow-Cytometry-NBP2-02576-img0001.jpg)
![Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody (NBP1-48303) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3C12-H11-Western-Blot-NBP1-48303-img0007.jpg "Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody (NBP1-48303) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Immunohistochemistry analysis using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody (NBP1-48303) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Granular layer of the epidermis. Some dermal staining.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3C12-H11-Immunohistochemistry-NBP1-48303-img0008.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3C12-H11-Immunohistochemistry-Paraffin-NBP1-48303-img0003.jpg)
![Western Blot: HSPB8/HSP22 Antibody [NBP1-30922] - Sample (30 ug whole cell lysate) A:293T whole cell lysateB:A431 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Western-Blot-NBP1-30922-img0007.jpg "Western Blot: HSPB8/HSP22 Antibody [NBP1-30922] - Sample (30 ug whole cell lysate) A:293T whole cell lysateB:A431 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-30922] - Analysis of HeLa, using NBP1-30922 at 1:200 dilution.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30922-img0005.jpg)
![Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-30922] - Human lung SCC, using HSP22 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-Immunohistochemistry-Paraffin-NBP1-30922-img0006.jpg)
![Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3K10W1-Western-Blot-NBP3-15464-img0003.jpg "Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Immunofluorescence analysis of U-2 OS cells using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3K10W1-Immunocytochemistry-Immunofluorescence-NBP3-15464-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Immunofluorescence analysis of NIH-3T3 cells using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3K10W1-Immunocytochemistry-Immunofluorescence-NBP3-15464-img0001.jpg)
![Western Blot: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Analysis of HSPB8 expression in HeLa(Cat # L013V1).](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3C5-Western-Blot-H00026353-M12-img0002.jpg "Western Blot: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Analysis of HSPB8 expression in HeLa(Cat # L013V1).")
![Sandwich ELISA: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Detection limit for recombinant GST tagged HSPB8 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-3C5-Sandwich-ELISA-H00026353-M12-img0001.jpg)
![Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M02), clone 5D7.Lane 1: HSPB8 transfected lysate(35.446 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-5D7-Western-Blot-H00026353-M02-img0004.jpg "Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M02), clone 5D7.Lane 1: HSPB8 transfected lysate(35.446 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Detection limit for recombinant GST tagged HSPB8 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-5D7-Sandwich-ELISA-H00026353-M02-img0003.jpg)
![Western Blot: HSPB8/HSP22 Antibody (5C3) [H00026353-M03] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M03), clone 5C3. Lane 1: HSPB8 transfected lysatE (35.446 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-5C3-Western-Blot-H00026353-M03-img0003.jpg "Western Blot: HSPB8/HSP22 Antibody (5C3) [H00026353-M03] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M03), clone 5C3. Lane 1: HSPB8 transfected lysatE (35.446 KDa). Lane 2: Non-transfected lysate.")
![ELISA: HSPB8/HSP22 Antibody (5C3) [H00026353-M03] - Detection limit for recombinant GST tagged HSPB8 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-5C3-ELISA-H00026353-M03-img0002.jpg)
![Western Blot: Hsp22 Overexpression Lysate (Adult Normal) [NBL1-11761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp22.](http://images.novusbio.com/fullsize/Hsp22-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11761-img0002.jpg "Western Blot: Hsp22 Overexpression Lysate (Adult Normal) [NBL1-11761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp22.")
![SDS-Page: Recombinant Human HSPB8/HSP22 Protein [NBP1-41159] - HSPB8/HSP22, 23.7 kDa (216aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/HSP22-Protein-SDS-Page-NBP1-41159-img0001.jpg "SDS-Page: Recombinant Human HSPB8/HSP22 Protein [NBP1-41159] - HSPB8/HSP22, 23.7 kDa (216aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")