![Western Blot: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hsp22.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-OTI1E3-Azide-and-BSA-Free-Western-Blot-NBP2-71828-img0002.jpg "Western Blot: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hsp22.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, CyTOF-ready |
| Clone | OTI1E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human HSPB8(NP_055180) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HSPB8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 21.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HSPB8/HSP22 Antibody (NBP2-71828)Find related products by research area.
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| Gene Symbol | HSPB8 |
![Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp22.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-OTI1E3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71828-img0005.jpg "Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp22.")
![Western Blot: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-OTI1E3-Azide-and-BSA-Free-Western-Blot-NBP2-71828-img0001.jpg "Western Blot: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.")
![CyTOF-ready: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - Analysis of Jurkat cells, using anti-Hsp22 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-OTI1E3-Azide-and-BSA-Free-CyTOF-ready-NBP2-71828-img0003.jpg "CyTOF-ready: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - Analysis of Jurkat cells, using anti-Hsp22 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![CyTOF-ready: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Hsp22 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/HSPB8-HSP22-Antibody-OTI1E3-Azide-and-BSA-Free-CyTOF-ready-NBP2-71828-img0004.jpg "CyTOF-ready: HSPB8/HSP22 Antibody (OTI1E3) - Azide and BSA Free [NBP2-71828] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Hsp22 antibody, and then analyzed by flow cytometry.")
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