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HSPB8/HSP22 Antibody (5D7)

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Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M02), clone 5D7.Lane 1: HSPB8 transfected lysate(35.446 KDa).Lane 2: Non-transfected lysate.
Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M02), clone 5D7.Lane 1: HSPB8 transfected lysate(35.446 KDa).Lane 2: ...read more
Sandwich ELISA: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Detection limit for recombinant GST tagged HSPB8 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
5D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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HSPB8/HSP22 Antibody (5D7) Summary

Immunogen
HSPB8 (NP_055180, 97 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT
Specificity
HSPB8 (5D7)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPB8
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSPB8/HSP22 Antibody (5D7)

  • Alpha-crystallin C chain
  • CMT2L
  • CRYAC
  • DHMN2
  • E2IG1
  • E2IG1E2-induced gene 1 protein
  • H11
  • H11DHMN2
  • heat shock 22kDa protein 8
  • heat shock 27kDa protein 8
  • heat shock protein beta-8
  • HMN2
  • HMN2A
  • HSP22
  • HSP22CMT2L
  • HSPB8
  • Protein kinase H11
  • Small stress protein-like protein HSP22

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HSPB8/HSP22
Antibody (5D7)
H00026353-M02
HSPB8/HSP22 Antibody (5D7)
Species: Hu
Applications: WB, ELISA

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Bioinformatics

Gene Symbol HSPB8
Entrez
OMIM
Uniprot