HSPB8/HSP22 Antibody (3C12-H11) Summary
Immunogen |
His-tagged human recombinant HSP22 |
Localization |
Cytoplasm , Nucleus |
Specificity |
Detects approx 22kDa. Does not cross react with alpha crystallin. |
Isotype |
IgG1 Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
HSPB8 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry 1:100
- Immunohistochemistry-Paraffin 1:10-1:500
- Western Blot 1:2000
|
Application Notes |
1 ug/ml of HSP22 Antibody was sufficient for detection of HSP22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4), 50% Glycerol |
Preservative |
0.09% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for HSPB8/HSP22 Antibody (3C12-H11)
Background
Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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