HSPB8/HSP22 Antibody (3C12-H11)

Images

 
Western Blot: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Load: 15 ...read more
Immunohistochemistry: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Immunohistochemistry analysis using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11 (NBP1-48303). Tissue: backskin. Species: Mouse. Fixation: ...read more
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody (3C12-H11) [NBP1-48303] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3C12-H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSPB8/HSP22 Antibody (3C12-H11) Summary

Immunogen
His-tagged human recombinant HSP22
Localization
Cytoplasm , Nucleus
Specificity
Detects approx 22kDa. Does not cross react with alpha crystallin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPB8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000
Application Notes
1 ug/ml of HSP22 Antibody was sufficient for detection of HSP22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSPB8/HSP22 Antibody (3C12-H11)

  • Alpha-crystallin C chain
  • CMT2L
  • CRYAC
  • DHMN2
  • E2IG1
  • E2IG1E2-induced gene 1 protein
  • H11
  • H11DHMN2
  • heat shock 22kDa protein 8
  • heat shock 27kDa protein 8
  • heat shock protein beta-8
  • HMN2
  • HMN2A
  • HSP22
  • HSP22CMT2L
  • HSPB8
  • Protein kinase H11
  • Small stress protein-like protein HSP22

Background

Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-32972
Species: Ch, Pm, Hu, Pm, Mu, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF4200
Species: Rt
Applications: WB
NBP1-47708
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-81696
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NLS3775
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP2-07996
Species: Hu
Applications: WB
NBP2-52486
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-92794
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00027129-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP2-20154
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-47752
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, WB
H00003166-B01P-50ug
Species: Hu
Applications: WB
AF2584
Species: Mu
Applications: Simple Western, WB
NBP1-48303
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC

Publications for HSPB8/HSP22 Antibody (NBP1-48303) (0)

There are no publications for HSPB8/HSP22 Antibody (NBP1-48303).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSPB8/HSP22 Antibody (NBP1-48303) (0)

There are no reviews for HSPB8/HSP22 Antibody (NBP1-48303). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSPB8/HSP22 Antibody (NBP1-48303) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HSPB8/HSP22 Products

Research Areas for HSPB8/HSP22 Antibody (NBP1-48303)

Find related products by research area.

Blogs on HSPB8/HSP22

There are no specific blogs for HSPB8/HSP22, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSPB8/HSP22 Antibody (3C12-H11) and receive a gift card or discount.

Bioinformatics

Gene Symbol HSPB8
Uniprot